Figure 3

Cell adhesion and proliferation of the WA17 cell line model of DS. (A) Plates were coated with FN (5 μg/ml) or Col VI (10 μg/ml). A9 (open bars) and WA17 (gray filled bars) cell lines (n = 12 independent cultures for each cell line and each ECM condition) were allowed to adhere to the protein coated plates for 60 minutes. Adhesion assays were performed as described in Materials and Methods. The percentage of adherent cells are shown as mean ± SD. (**p < 0.001 T-test comparing FN and Col VI conditions for WA17). (B) Proliferation assay was performed as described in Materials and Methods, on plates coated with ColVI and FN, as above. Data represent the means ± SD (*p < 0.01 T-test comparing FN and Col VI conditions for WA17).