Figure 2
From: Proteomic analysis of total cellular proteins of human neutrophils
Total extract of resting human neutrophils separated by 1D SDS-PAGE. The protein extract was submitted to SDS-PAGE separation, stained with Coomassie Blue and cut into 20 horizontal bands. Each gel band was hydrolyzed with trypsin and the tryptic peptides were analyzed by LC-MS/MS. Lane 1: molecular weight standards (kDa); Lanes 2 and 3: 50 μg in duplicate of human neutrophil extract.