Analysis of the urine proteome. (A) In Workflow C, pooled urine samples were labelled and resolved by 2DGE. (1) DIGE image of proteins from pools of healthy normals and hepatitis E patients labelled with Cy3 and Cy5, respectively. (2) DIGE image of proteins from pools of hepatitis E patients and healthy normals labelled with Cy3 and Cy5, respectively. (B) DIGE image of urine proteins from acute mild hepatitis patient (H3) labelled with Cy3 and those from a case of fulminant hepatitis E (H4) labelled with Cy5. Box 1 shows proteins spots that were downregulated in fulminant hepatitis compared to acute mild infection, while Box 2 shows proteins that remained unchanged between the two forms of disease.