Effects of UVB-induced oxidative stress on protein expression and specific protein oxidation in normal human epithelial keratinocytes: a proteomic approach

Table 2 Proteins with increased oxidation after UVB irradiation of NHEK cells.

Spot n°	Protein name	Fold	Theorethical mw/pI	Sequence Coverage %	Protein Score	P value
1	Glucosidase 2 β subunit	4.2	60357/4.33	16	131	<0.002
2	GRP 78	4.5	72402/5.07	12	80	<0.02
3	Heterogeneous nuclear ribonucleoproteins C1/C2	1.55	33707/4.95	30	67	<0.04
4	Protein disulfide-isomerase A3	4.2	57146/5.98	42	226	<0.002
5	Actin-related protein 3	11.6	47797/5.61	19	110	<0.015
6	α-enolase	3.4	47481/7.01	31	112	<0.02
7	Annexin 2	5.1	36201/8.57	56	104	<0.02

For each protein the carbonyl immunoreactivity/protein expression values were averaged (n = 6) and expressed as fold increase in irradiated cells compared to control.

ISSN: 1477-5956