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Table 1 Comparison of MALDI efficiency for the workflows with two databanks

From: A gel-free approach in vascular smooth muscle cell proteome: perspectives for a better insight into activation

workflow

MS Spectraa)

MSMS Spectrab)

Identified

peptides

MSMS

efficiency(%)c)

Proteinsd)

peptides/proteins

Proteins by single peptidee)

DataBankf)

1 (Anion)

597

6868

3530

51%

658

5.4

141

SwissProt (Mammalia)

2 (Cation)

597

6212

2705

43%

335

8.1

139

SwissProt (Mammalia)

1 (Anion)

597

6868

4232

62%

900 (555Pred)

4.07

53

NCBI

(Sus Scrofa)

2 (Cation)

597

6212

3317

54%

720 (467Pred)

4.07

34

NCBI

(Sus Scrofa)

  1. a) number of total MS spectra/workflow; b) number of total MSMS spectra/workflow; c) efficiency is determined dividing the number of identified peptides per MSMS spectra; d) number of unique identified proteins. "Pred" is an abbreviation for "Predicted" e) number of proteins identified by a single peptide as reported by GPS Explorer software; f) database chosen in protein identifications in GPS Explorer software.