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Figure 2 | Proteome Science

Figure 2

From: Downregulated expression of HSP27 in human low-grade glioma tissues discovered by a quantitative proteomic analysis

Figure 2

One pair of isotope peptides (m/z, 582/581) "EITA L APSTMK" of β-actin was chosen to be an internal standard to validate the mixture ratio. (A) showed the peak intensity of the isotope-labeling peptides from the protein mixture containing human glioma tissues (HGTs) and H4 cells. The SILAC ratio1 of HGTs versus labeled H4 cells was 1.03 ± 0.02. (B) represented the peak intensity of isotope-labeling peptides, which came from the protein mixture from para-cancerous brain tissues (PBTs) and labeled H4 cells. The SILAC ratio2 of PBTs versus labeled H4 cells was 0.98 ± 0.03. Thus, the change ratio of β-actin in HGTs versus PBTs was 1.05, near 1:1, which indicated the expression of β-actin was similar between the two tissues.

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Proteome Science

ISSN: 1477-5956