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Table 1 Proteins no longer expressed after the 2nd culture passage.

From: Proteome analysis of human Wharton's jelly cells during in vitro expansion

Spot # Abbr. Name ACa Protein Name Scoreb SCc % pI Mr(Da) Theo. pI Mr(Da) Exp.
2 PAI2 P05120 Plasminogen activator inhibitor 2 123 46 5,46 46596 5,44 30700
22 SHOT1 A0MZ66 Shootin 1 56 39 5,27 72109 4,80 50629
34 EIF3I Q13347 Eukaryotic translation initiation factor 3 subunit I 214 61 5,38 36502 5,21 34955
35 ACTG P63261 Gamma Actin 68 32 5,31 41792 4,98 32910
36 ACTB P60709 Beta Actin 96 58 5,29 41736 5,04 33210
61 KAD5 Q9Y6K8 Adenylate kinase isoenzyme 5 81 37 5,23 22420 4,80 28831
67 VIME P08670 Vimentin 100 35 5,06 53651 4,95 47492
75 KAD5 Q9Y6K8 Adenylate kinase isoenzyme 5 79 54 5,23 22420 4,70 27829
  1. A) Entry code number from SWISS PROT database.
  2. B) Score is -10*Log(P), where P is the probability that the observed match is a random event, it is based on Swiss-Prot database using the MASCOT searching program as MALDI-TOF data.
  3. C)Sequence coverage means the ratio of portion sequence covered by matched peptide to the full length of the protein sequence.