Figure 2

Complementary western blot analysis of novel PARP-1 and PARP-2 interactors. A and B) Immunoprecipitation of PARP-1 and associated proteins. In A) immunodetection of PARP-1 (bait) and the novel interactors Btf, identified by AP-MS. In B) immunodetection of novel PARP-1 interactors FMR-1 and AIF, absent from AP-MS. C and D) Immunoprecipitation of PARP-2 and associated proteins. In A) immunodetection of PARP-2 (bait) and the novel interactors KU70, KU80 and FMR-1, identified by AP-MS. In B) immunodetection of novel PARP-2 interactors BTF, AIF and STAT-1, absent from AP-MS. Each immunodetection is done in whole cellular extract (WCE) and immunoprecipitates (IP) from control (CTRL) and the bait (PARP-1 or FLAG-PARP-2).