Figure 1

Hierarchical clustering of Arabidopsis thaliana ecotypes based on 2DE spot amounts. Soluble proteins from ecotype Col-0 were separated using pH 4-7 IPG in first dimension and 11% SDS-PAGE in second dimension. Proteins spots were visualized by colloidal Coomassie blue staining. The amount of each spot was estimated by its normalized volume as obtained by image analysis [62]. Euclidian distances were then computed for all spots to build the similarity matrix for ecotypes, and clustering was performed using the Ward's method to link the variables.