Figure 3
From: Highlights on the capacities of "Gel-based" proteomics
Characterization of isoforms of alpha-amylase in human saliva. A 2DE map of human salivary proteins was performed [68]. Proteins were resolved using pH 3-10NL IPG and 12% SDS-PAGE, and stained with colloidal Coomassie blue. Alpha-amylase spots subsequently identified by MALDI-TOF MS are indicated by respective spot number (left part). According to the mass features of alpha-amylase (right part) identified spots, (A) coverage of the alpha-amylase sequence by the total population of peptides identified (black boxes) in the different alpha-amylase spots, (B) simultaneous clustering of the 67 alpha-amylase spots according to the MW range measured on gels, the MS identification of peptides in the N-terminal and C-terminal and central regions of the sequence, (C) individual spot coverage of the alpha-amylase sequence by peptides identified (black boxes) by MALDI-TOF MS.