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Figure 3 | Proteome Science

Figure 3

From: Development of high-yield autofluorescent protein microarrays using hybrid cell-free expression with combined Escherichia coli S30 and wheat germ extracts

Figure 3

Analysis of cell-free expression levels obtained using a wheat germ extract. Microarrays were scanned at 488 nm using the MDS GenePix Autoloader 4200AL. Left Panel: Fluorescence is detectable above background for most templates, but high levels of fluorescence are observed only for sub-array A2, representing the positive control (purified GST-GFP in Stabilguard). The microarray was scanned twice, the first time for the purposes of element quantification, and the second time, with the gain settings increased, to allow visualization of low-level expression; this results in saturation of the image in sub-array A2. Right Panel: The amounts of fluorescence were quantified from the scanned images, and the data presented in the order of sub-arrays A1, A2, A3, B1.....E1, E2, E3.

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Proteome Science

ISSN: 1477-5956