Development of high-yield autofluorescent protein microarrays using hybrid cell-free expression with combined Escherichia coli S30 and wheat germ extracts

Zárate, Xristo; Henderson, David C; Phillips, Keenan C; Lake, April D; Galbraith, David W

doi:10.1186/1477-5956-8-32

Proteome Science

Table 1 Proteins expressed on autofluorescent protein microarrays via hybrid cell-free expression

From: Development of high-yield autofluorescent protein microarrays using hybrid cell-free expression with combined Escherichia coli S30 and wheat germ extracts

DNA/Protein	[Swiss-Prot]	Reference	Locus ID	Clone*	Size (aa)	Fluorescence Intensity (a.u.)	CV ⁺
Ankyrin-repeat	Q9FF09	[59]	At5g07840	U14849	175	57167	13.1
MBP	P0AEX9	[31]			368	53806.6875	14.5
GFP	P42212	[26]			242	48381.375	11.9
IMB1	Q56W05	[60]	At2g34900	pda11561	386	37860.1875	14.8
IAA9	Q38827	[61]	At5g65670	C00016(E)	338	30984.0625	7.8
AXR3	P93830	[62]	At1g04250	pda03374	229	29581.875	8.2
SHY2	Q38822	[63]	At1g04240	C00011(F)	189	28544.3125	9.7
GST	P26624	[32]			224	26364.8125	12.9
PIF3	O80536	[64]	At1g09530		524	21759.5625	17.3
PIL5	Q8GZM7	[65]	At2g20180		407	19228.25	19.3
ELF3	O82804	[66]	At2g25930	U24077	339	16343.8125	9.8
PP2C	Q9LME4	[59]	At1g22280	U19121	281	10684.125	12.5
FYPP3	Q9LHE7	[67]	At3g19980	U21104	303	8129.5	9.5
PixE	P74294	[34]			380	5983.25	10.4
TUB1	Q8GZ16	[68]	At1g75780	pda10291	447	4330.3125	9.9
PKS1	Q9SWI1	[69]	At2g02950	U13787	439	3335.0625	9.9
LovR	Q9ABE4	[33]			152	3312.25	9.4
CRY1	Q43125	[70]	At4g08920	U12079	681	3084.25	8.8
CCA1	Q8S8N5	[71]	At2g46830	C105127	526	2739.6875	4.7
NACA2	Q94JX9	[59]	At3g49470	U15737	217	2218.5625	7.1
NDPK2	O64903	[72]	At5g63310	U19177	231	2209.75	8.7
PIL6	Q84LH8	[73]	At3g59060	U16079	300	2166.125	5.4
GID1L1	Q9MAA7	[74]	At3g05120	U17384	345	2143.1875	6.7
FHY1	Q8S4Q6	[75]	At2g37678		202	2123.8125	3.1
DET2	Q38944	[76]	At2g38050	U09847	262	1980.8125	3.7
EID1	Q8LEA8	[77]	At4g02440	pda12208	336	1882.9375	7
PCL1	Q9SNB4	[78]	At3g46640	U22928	323	1821	5.6
SLY2	Q9LUB6	[79]	At5g48170	S63202	157	1747.3125	7.8
CRY2	Q96524	[80]	At1g04400	U19559	612	1715.1875	14.4
RGL1	Q9C8Y3	[81]	At1g66350	U18422	511	1320.0625	10.5
RGA	Q9SLH3	[82]	At2g01570	U13937	587	1286.75	9
No expression
DNA/Protein
GAI	Q9LQT8	[74]	At1g14920	U14047	533	1190.4375	2.8
RGL3	Q9LF53	[83]	At5g17490	U60167	523	1139.1875	1.6
TOC1	Q9LKL2	[84]	At5g61380	U21896	618	1093.5625	3.6
SEC	Q9M8Y0	[85]	At3g04240		977	1091.0625	4.1
SPY	Q96301	[86]	At3g11540		914	1054.75	3.4
No DNA						1027.9375	3.9

*Indicates the cDNA clone used to produce T7 promoter-based DNA constructs for protein expression. Clone names that start with 'pda' are from the RIKEN Bioresource Center, Japan; those starting with U or C were purchased from the Arabidopsis Biological Resource Center, Columbus, OH. If no clone is indicated, the cDNA was obtained from an individual researcher.
⁺CV: coefficient of variation.

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ISSN: 1477-5956

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