Application of highly sensitive saturation labeling to the analysis of differential protein expression in infected ticks from limited samples

Table 2 Summary of differentially expressed proteins in the comparative analysis of Rhipicephalus spp. adult female ticks naturally infected with different pathogens.

Tick/pathogen	Total numberof differentially expressed protein spots	Under expressedin infected ticks	Over expressedin infected ticks
R. sanguineus/R. conorii	65	48	17
R. sanguineus/E. canis	59	41	18
R. bursa/T. annulata	54	37	17
R. turanicus/A. ovis	50	20	30

Ticks infected with different pathogens were compared with their respective uninfected controls and the proteins that were differentially expressed with an average ratio of ± 5-fold after DeCyder software analysis of DIGE gels were considered. Two experiments were conducted with similar results.

ISSN: 1477-5956