Application of highly sensitive saturation labeling to the analysis of differential protein expression in infected ticks from limited samples

Table 4 Comparative analysis between protein and mRNA levels for selected differentially expressed tick proteins.

		Infected/Uninfected
Tick protein	Tick/pathogen	Protein	mRNA
Unknown larval protein
	R. sanguineus/E. canis	Down	Up
	R. sanguineus/R. conorii	Unchanged	Unchanged
	R. bursa/T. annulata	Down	Unchanged
	R. turanicus/A. ovis	Up	Down
Guanine nucleotide-binding protein
	R. sanguineus/R. conorii	Down	Unchanged
	R. sanguineus/E. canis	Unchanged	Unchanged
	R. bursa/T. annulata	Down	Down
	R. turanicus/A. ovis	Down	Down

The mRNA levels of the differentially expressed tick guanine nucleotide-binding and unknown larval proteins were analyzed by real-time RT-PCR with RNAs from individual infected and uninfected ticks and correlated with protein levels determined by 2-D DIGE.

ISSN: 1477-5956