Figure 2
From: Comparative analysis of proteome maps of silkworm hemolymph during different developmental stages
Two-dimensional electrophoresis of silkworm hemolymph at different stages. Male(♂) and female(♀) hemolymph sample were subjected to 2D-electrophoresis separately. Protein (60 μg) was separated by IEF using 13 cm IPG strips (pH 3-10), followed by 12.5% SDS-PAGE and silver-staining. L3: 3rd day fifth instar larva; L6: 6th day fifth instar larval; P3: 3rd day pupa; P8: 8th day pupa; M0: eclosion. Circles indicate 56 spots excised for MALDI-TOF MS, most of which had different expression during development and metamorphosis.