Figure 5
From: Comparative analysis of proteome maps of silkworm hemolymph during different developmental stages
Identification of spot 8 from 3rd day of pupation by peptide mass fingerprint and MS/MS. Spot 8 was excised from gels and digested with trypsin, and the resulting peptides were analyzed using MALDI-TOF mass spectrometry and TOF/TOF mass spectrometer. (A) MALDI-TOF MS on in-gel-digested peptides of spot 8. A total of 22 deisotoped peptide masses were submitted to a database search, and 14 peptides matched two members of the 30 K protein family of silkworm. Arrows indicate peptides from Bmlp4. Arrowheads indicate peptides from Bmlp1. (B) MS/MS spectra of ion 1064.28 from Bmlp1. (C) MS/MS spectra of ion 1134.25 from Bmlp4. Fragment ion segments from the precursor ions confirmed the results of peptide mass fingerprint. The y and b ions and their corresponding peptide sequences are shown.