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Table 2 List of cytoplamic proteins of D. seriata.

From: Cytoplasmic- and extracellular-proteome analysis of Diplodia seriata: a phytopathogenic fungus involved in grapevine decline

Spot

Acc. number

Protein identity a

PM/Seq cov./score b

Identified by

Total Ion scorec

Experimental MW/pI

Theoretical MW/pI

Organism

1

gi|238498522

Phosphoglycerate kinase

12/30%/251

MASCOT

 

44.0/8.3

44.4/7.7

Aspergillus flavus

2

gi|67903236

Hypothetical protein (peptidyl-prolyl cis-trans isomerase or cyclophilin 1)

5/47%/219

MASCOT

 

18.2/8.5

17.8/8.9

Aspergillus nidulans

3

gi|169601412

Hypothetical protein (enolase)

11/30%/595

MASCOT

 

48.1/6.2

47.4/5.2

Phaeosphaeria nodorum

4

gi|198425409

Predicted Zn-finger U1-like

12/54%/88

MASCOT

 

12.3/4.9

22.6/9.5

Ciona intestinalis

5

gi|156053161

Hypothetical protein (aldehyde reductase)

2/5%/82

MASCOT

77

36.2/7.8

37.9/8.8

Sclerotinia sclerotiorum

6

gi|169625443

Hypothetical protein (triosephosphate isomerase)

7/23%/257

MASCOT

 

25.0/6.9

27.1/6.1

Phaeosphaeria nodorum

7

gi|46581334

Hypothetical protein

6/23%/112

MASCOT

 

30.2/7.4

16.8/6.3

Desulfovibrio vulgaris

8

gi|189210148

Signal transduction protein (CipC-like antibiotic response protein)

3/19%/117

MASCOT

 

13.1/4.8

14.9/4.9

Pyrenophora tritici-repentis

9

gi|115400267

Transaldolase

6/18%/165

MASCOT

 

35.2/5.2

35.2/6.2

Aspergillus terreus

10

gi|85107722

Hypothetical protein (phosphoglycerate kinase)

10/22%/217

MASCOT

 

45.2/7.9

45.3/6.2

Neurospora crassa

11

gi|120691

Glyceraldehyde-3-phosphate dehydrogenase

8/23%/334

MASCOT

 

38.3/8.2

36.6/6.7

Cochliobolus lunatus

12

gi|189204622

Fructose-bisphosphate aldolase

4/9%/156

MASCOT

140

38.4/6.7

40.2/5.5

Pyrenophora tritici-repentis

13

gi|189197705

Glycolipid transfer protein

5/25%/98

MASCOT

 

22.6/6.9

24.0/5.8

Pyrenophora tritici-repentis

14

gi|126139808

Hypothetical protein (thiamine biosynthesis enzyme)

5/20%/124

MASCOT

 

39.1/6.5

38.1/5.4

Pichia stipitis

15

gi|212542889

Glycerol dehydrogenase Gcy1 putative

4/17%/306

MASCOT

 

33.6/8.4

33.7/6.0

Aspergillus fumigatus

16

gi|67903236

Hypothetical protein (peptidyl-prolyl cis-trans isomerase or cyclophilin 1)

6/50%/214

MASCOT

 

18.5/7.9

17.8/8.9

Aspergillus nidulans

17

gi|70985178

Thiamine biosynthesis protein

8/25%/325

MASCOT

 

37.2/6.0

38.6/6.0

Aspergillus fumigatus

18

gi|255943905

Malate dehydrogenase

9/26%/471

MASCOT

 

34.6/7.9

35.8/8.4

Penicillium chrysogenum

19

gi|254583736

Hypothetical protein (fumarate reductase)

5/9%/161

MASCOT

144

56.9/6.7

54.0/8.6

Zygosaccharomyces rouxii

20

gi|154285406

Nucleoside-diphosphate kinase

6/38%/234

MASCOT

 

13.3/9.4

16.9/7.8

Ajellomyces capsulatus

21

gi|164429080

Adenosine kinase

5/12%/252

MASCOT

236

38.1/5.3

49.4/6.0

Neurospora crassa

22

gi|218722748

Conserved hypothetical protein

4/19%/157

MASCOT

 

51.4/4.9

42.8/5.8

Talaromyces stipitatus

23

gi|189196154

NADP-specific glutamate dehydrogenase

10/22%/504

MASCOT

 

46.4/7.5

49.1/5.9

Pyrenophora tritici-repentis

24

gi|58265982

Eukaryotic translation initiation factor 5A-1

5/30%/391

MASCOT

 

20.0/4.9

17.9/5.3

Cryptococcus neoformans

25

gi|145613040

Conserved hypothetical protein (mitochondrial peroxiredoxin PRX1)

4/21%/306

MASCOT

 

25.4/6.6

25.2/5.7

Magnaporthe grisea

26

gi|225877962

Putative saccharopine dehydrogenase

4/11%/146

MASCOT

131

45.2/5.6

43.5/5.2

Gibberella fujikuroi

27

gi|194669270

Endonuclease reverse transcriptase

23/24%/88

MASCOT

 

37.8/6.8

150.3/9.7

Bos taurus

28

gi|169625443

Hypothetical protein (triosephosphate isomerase)

5/19%/178

MASCOT

 

25.4/6.4

27.1/6.1

Phaeosphaeria nodorum

29

gi|255711238

KLTH0B02596p

9/70%/73

MASCOT

 

39.5/6.0

16.3/5.4

Lachancea thermotolerans

30

gi|154318339

Hypothetical protein (Elongation factor 1β)

2/20%/87

PRO-BLASTd

 

35.2/4.1

17.7/4.7

Botryotinia fuckeliana

31

gi|67903236

Hypothetical protein (peptidyl-prolyl cis-trans isomerase or cyclophilin 1)

5/35%/168

MASCOT

 

19.0/8.0

17.8/8.9

Aspergillus nidulans

32

gi|189196154

NADP-specific glutamate dehydrogenase

11/29%/541

MASCOT

 

46.4/7.2

49.1/5.9

Pyrenophora tritici-repentis

33

gi|145613040

Conserved hypothetical protein (mitochondrial peroxiredoxin PRX1)

4/21%/305

MASCOT

 

26.0/6.5

25.2/5.7

Magnaporthe grisea

34

gi|156057169

ATP sulfurylase

11/22%/98

MASCOT

 

74.6/4.4

64.2/6.6

Sclerotinia sclerotiorum

35

gi|169596136

Hypothetical protein (piruvate kinase)

12/28%/271

MASCOT

 

72.8/6.8

58.4/5.7

Phaeosphaeria nodorum

36

gi|154315126

S-Adenosylmethionine synthetase

5/18%/136

MASCOT

 

43.9/5.8

58.4/5.7

Botryotinia fuckeliana

37

gi|148361511

Vacuolar serine protease

7/20/115

MASCOT

 

15.7/6.1

41.0/5.8

Cladosporium cladosporioides

38

gi|85094513

Hypothetical protein (phosphoglyceromutase)

9/17/222

MASCOT

 

64.5/6.1

57.0/5.4

Neurospora crassa

39

gi|145232889

Hypothetical protein (pyruvate decarboxylase)

6/18/123

MASCOT

 

75.1/5.3

63.0/6.3

Aspergillus niger

40

gi|116196014

Hypothetical protein (Heat shock 70 kDa protein, mitochondrial)

12/20/369

MASCOT

 

84.8/5.4

68.2/5.6

Chaetomium globosum

41

gi|258575103

3'(2'),5'-Bisphosphate nucleotidase

10/25/335

MASCOT

 

41.5/5.6

38.1/5.0

Uncinocarpus reesii

42

gi|258577135

Rab GPD dissociation inhibitor alpha

9/25/396

MASCOT

 

61.8/5.5

52.0/5.7

Uncinocarpus reesii

43

gi|255936655

Pc13g09300 (pyruvate decarboxylase)

7/19/107

MASCOT

 

75.4/5.4

63.3/5.7

Penicillium chrysogenum

44

gi|114321348

Peptidoglycan synthetase FtsI

11/17/93

MASCOT

 

30.6/6.9

62.7/8.0

Alkalilimnicola ehrlichii

45

gi|39945784

Hypothetical protein (UDP-glucose 4-epimerase Gal10)

5/15/344

MASCOT

323

39.8/6.6

40.9/5.9

Magnaporthe grisea

46

gi|6647556

3-Isopropylmalate dehydrogenase A

5/16/198

MASCOT

 

41.4/5.8

38.8/5.7

Aspergillus niger

47

gi|74627960

Mannitol-1-phosphate 5-dehydrogenase

7/17/191

MASCOT

 

45.7/5.7

43.7/6.0

Alternaria alternata

48

gi|255942351

Hypothetical protein (UDP-galactoyranose mutase)

9/21/250

MASCOT

 

60.1/7.0

57.2/5.6

Penicillium chrysogenum

49

gi|145232889

Hypothetical protein (pyruvate decarboxylase)

6/18/118

MASCOT

 

74.0/5.9

63.0/6.3

Aspergillus niger

50

gi|576627

Alcohol dehydrogenase

4/16/240

MASCOT

 

36.0/8.2

37.6/6.4

Candida albicans

51

gi|255933774

Pc12g14620 (flavohemoprotein)

04/08/1972

PRO-BLASTe

 

42.5/7.5

46.0/6.0

Penicillium chrysogenum

52

gi|261359248

Aldose reductase

02/05/1991

PRO-BLASTf

 

37.0/7.5

33.6/5.4

Verticillium albo-atrum

53

gi|242812056

Alcohol dehydrogenase

04/06/1999

PRO-BLASTg

 

36.1/8.1

38.9/6.0

Talaromyces stipitatus

  1. aThe assignment of the biological or enzymatic function to the hypothetical proteins (based on BlastP analyses) is shown between parentheses and in italic characters.
  2. bNumber of peptides matched/Sequence coverage (%)/Score
  3. cTotal and best ion scores are shown for proteins showing a sequence coverage value less than 15%
  4. dTwo best peptides (PRO-BLAST): SYLVGYASPKADV/APDAAKFPHAAR
  5. eTwo best peptides (PRO-BLAST): RDPIIAEHGNTITTR/SMAPHINHIAATRNPVR
  6. fTwo best peptides (PRO-BLAST): DIGTDYVDLYLIHDAKKTGGR/ALGLSNFSR
  7. gTwo best peptides (PRO-BLAST): EFPANEDHIAFAAR/KIVHPPKVR