Table 1 Differentially expressed proteins in femoral marrow of chickens within 12 weeks and 19 weeks after methylprednisolone (MP) induction (pooled sample of 4 chickens), compared with control subjects (pooled sample of 3 chickens).
Spot no. | Protein name | % coverage (score) | Swiss Prot. Accession no. | Matched species | Function | LF in 12th weeks | LF in 19th weeks |
|---|---|---|---|---|---|---|---|
1 | Coiled-coil domain-containing protein 43 | 41% (66) | Q5ZK95 | Chicken | Evidence at transcript level. | 0.9 | 1.4 |
2 | Cell death activator CIDE-B | 33% (69) | O70303 | Mouse | Activates apoptosis [31] | 1.1 | 2.1 |
3 | Uncharacterized protein C3orf59 | 17% (61) | Q8IYB1 | Human | Unknown | 1.1 | 2.0 |
4 | Haptoglobin precursor | 31% (64) | P00738 | Human | Combines with free plasma hemoglobin, preventing loss of iron | 1.4 | 1.7 |
5 | Serum amyloid P-component precursor | 26% (79) | P02743 | Human | May be involved in transcriptional regulation [12]. | 1.3 | 3.1 |
6 | Zinc finger protein 28 | 23% (61) | P17035 | Human | May be involved in transcriptional regulation. | 1.2 | 4.1 |
7 | Endothelial zinc finger protein 71 | 28% (57) | Q9NQZ8 | Human | May be involved in transcriptional regulation. | 1.2 | 5.6 |
8 | T-box transcription factor 3 TBX3 | 12% (59) | Q7TST9 | Chicken | Transcriptional repressor involved in developmental processes. Probably plays a role in limb pattern formation [9]. | 1.0 | 6.2 |
9 | Cyclin-dependent kinase inhibitor 1 | 28% (62) | P39689 | Mouse | May be the important intermediate by which p53 mediates its role as an inhibitor of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression [32]. | 0.9 | 7.4 |
0 | Uncharacterized protein UNQ1940PRO4423 precursor | 27% (60) | Q6UWF9 | Human | Evidence at transcript level. | 0.8 | 7.5 |
11 | Uncharacterized protein C12orf52 homolog | 39% (66) | Q2HJ75 | Bovine | Evidence at transcript level. | 0.9 | 6.6 |
12 | Myosin | 23% (85) | Q17R14 | Bovine | Evidence at transcript level. | 1.5 | 7.4 |
13 | Dimethylaniline monooxygenase | 19% (75) | Q8K4B7 | Rat | This protein is involved in the oxidative metabolism of a variety of xenobiotics such as drugs and pesticides [29, 34] | 2.6 | 7.6 |