Figure 4
CRMP-2-ΔC in the brains of C57BL/6J mice during the progression of prion disease. (A) Western blot analysis for CRMP-2, GFAP and GAPDH. The accumulation of PK-resistant PrPSc is shown at the bottom. p: prion-infected; m: mock controls. The ladders of GFAP bands are possibly due to post-translational cleavage [24] or alternative splicing [51].
(B) The amounts of 61-kDa and 56-kDa CRMP-2 relative to the total amount of CRMP-2 as determined from the signal intensity of the bands in panel (A). Data are means ± SEM (n = 2).
(C) Northern blot analysis for prion-infected and mock-infected mice sacrificed at 160 dai (n = 2, respectively). The arrowhead indicates the CRMP-2 transcript. The ratios of signal intensities of the bands of CRMP-2 mRNA to that of GAPDH mRNA are shown after normalization to the ratio obtained from the control mice as 1.00 (means ± SEM).