Identification and structural analysis of C-terminally truncated collapsin response mediator protein-2 in a murine model of prion diseases

Table 1 Table 1 Summary of the mass spectrometry analysis

	2-DE^b)		Mass spectrometry						Theoretical^b)		Fold change^d)(160dai)	_pe)
Spot ^a)	Mr _obs (kDa)	pI _obs	Method	Identity	UniProt KB accession	Coverage	Number of peptides	Mowse score ^c)	Mr _cal (kDa)	pI _cal
a	56	5.45	MALDI & LC-ESI	CRMP-2-ΔC	O08553	39	17	963	56.5^f)	5.48^f)	7.96	0.076
b1	61	5.77	MALDI & LC-ESI	CRMP-2	O08553	45	22	1419	62.3^g)	5.95^g)	0.53	0.375
b2	61	5.72	MALDI & LC-ESI	CRMP-2	O08553	8	4	61	62.3	5.95^h)	0.39	0.249
b3	61	5.68	MALDI & LC-ESI	CRMP-2	O08553	7	4	103	62.3	5.95^h)	0.51	0.350
1	43	5.1	MALDI	GFAP	P03995	39	13	79	49.9	5.28	0.85	0.823
2	41	5.02	MALDI	GFAP	P03995	48	18	155	49.9	5.28	1.33	0.628
3	40	4.85	MALDI	GFAP	P03995	25	9	66	49.9	5.28	8.93	0.056
4	27	7.4	LC-ESI	Glutathione S- transferase μ1	P10649	21	7	176	25.8	8.14	+++ ⁱ⁾	ND
5	26	5.79	MALDI	Peroxiredoxin -6	O08709	44	8	121	24.7	5.72	1.58	0.389
6	24	5.02	LC-ESI	Peroxiredoxin -2	Q61171	38	8	372	21.6	5.2	0.90	0.850

a) The spots shown in Figure 1.
b) Mr_obs and Mr_cal, observed and theoretical Mr; pI_obs and pI_cal, observed and theoretical pI, respectively.
c) Probability-based Mowse score [52] calculated using MASCOT software [48].
d) Fold change of spot volume on 160 dai.
e) p-Value of Student's t-test. ND: not determined.
f) Mr_cal and pI_cal of non-phosphorylated CRMP-2^1-517.
g) Mr_cal and pI_cal of non-phosphorylated CRMP-2^1-572.
h) An approximate shift of -0.05 per phosphate moiety is expected [25, 26].
i) Undetectable in the mock sample.

ISSN: 1477-5956