Table 1 Pairing and annotating Haliotis asinina ESTs with shell derived MS/MS peptides
From: Proteomic analysis of the organic matrix of the abalone Haliotis asinina calcified shell
Protein name [GenBank Accession] | EST library* | Homology/ domain | Mass kDa (Observed/ Theoretical) | Complete sequence/ SignalP | Source of material | Best MASCOT protein scores | Number of matching peptides | ||
|---|---|---|---|---|---|---|---|---|---|
Band ID | Nacre | Pr + Nc | |||||||
ML5A7 [DW986289] | 1, 2 | No homology/no recognised domains | 32/25 | Yes/Yes | S6 I7 | - - | ASM AIM | 459 | 9 |
HasCL10contig2 [EZ420619] | 3 | Fibroin-like/Gly and Ala rich domains | -/44 | Yes/Yes | - - | ASM AIM | ASM AIM | 455 | 7 |
P0012N13_463 [GT274423] | 3 | Papilin/2 Kunitz domains | 14/≥15 | No/No | S3 I4 | ASM AIM | ASM AIM | 362 | 6 |
ML1E6 [DW986219] | 1, 2 | Sometsuke/ependymin domain | 32/20 | Yes/Yes | S6 I7 | - - | ASM AIM | 347 | 7 |
P0006O07_675 [GT272916] | 3 | No homology/no recognised domains | 13-48/≥19 | No/No | S2-S7 I3-I9 | ASM AIM | ASM AIM | 343 | 6 |
P0025F23_658 [GT276990] | 3 | No homology/Asp and Ala rich domains | 32/≥23 | No/No | - I7 | - AIM | - AIM | 343 | 3 |
ML3A11 [DW986237] | 1, 2, 3 | No homology/Pro rich domain | 15/16 | Yes/Yes | S3 I4 | ASM AIM | ASM AIM | 336 | 4 |
ML6A10 [DW986342] | 1, 2, 3 | No homology/no recognised domains | 13/12 | Yes/Yes | S2 I3 | ASM AIM | ASM AIM | 320 | 5 |
ML8B1 [DW986463] | 1, 2, 3 | No homology/Gln rich domains | 13/11 | Yes/Yes | S2 I3 | ASM AIM | ASM AIM | 195 | 3 |
P0011O14_517 [GT274178] | 3 | No homology/no recognised domains | 18/≥16 | No/Yes | - I5 | - AIM | - AIM | 189 | 3 |
6G3 [GD272908] | 2 | No homology/ependymin domain | 21/21 | Yes/Yes | S5 I6 | ASM AIM | ASM AIM | 173 | 3 |
ML3E9 [DW986256] | 1,2, 3 | Perlwapin/3 WAP domains | -/≥15 | No/No | - - | - - | ASM AIM | 148 | 3 |
ML5B8 [DW986296] | 1, 2 | No homology/no recognised domains | 32/21 | Yes/Yes | S6 - | - - | ASM | 125 | 2 |
ML5H8 [DW986339] | 1, 2, 3 | No homology/Chitin-Binding domain | -/10 | Yes/Yes | - - | - - | ASM AIM | 116 | 3 |