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Table 1 Pairing and annotating Haliotis asinina ESTs with shell derived MS/MS peptides

From: Proteomic analysis of the organic matrix of the abalone Haliotis asinina calcified shell

Protein name [GenBank Accession] EST library* Homology/
domain
Mass kDa (Observed/
Theoretical)
Complete sequence/
SignalP
Source of material Best MASCOT
protein scores
Number of matching peptides
      Band ID Nacre Pr + Nc   
ML5A7
[DW986289]
1, 2 No homology/no recognised domains 32/25 Yes/Yes S6
I7
-
-
ASM
AIM
459 9
HasCL10contig2
[EZ420619]
3 Fibroin-like/Gly and Ala rich domains -/44 Yes/Yes -
-
ASM
AIM
ASM
AIM
455 7
P0012N13_463
[GT274423]
3 Papilin/2 Kunitz domains 14/≥15 No/No S3
I4
ASM
AIM
ASM
AIM
362 6
ML1E6
[DW986219]
1, 2 Sometsuke/ependymin domain 32/20 Yes/Yes S6
I7
-
-
ASM
AIM
347 7
P0006O07_675
[GT272916]
3 No homology/no recognised domains 13-48/≥19 No/No S2-S7
I3-I9
ASM
AIM
ASM
AIM
343 6
P0025F23_658
[GT276990]
3 No homology/Asp and Ala rich domains 32/≥23 No/No -
I7
-
AIM
-
AIM
343 3
ML3A11
[DW986237]
1, 2, 3 No homology/Pro rich domain 15/16 Yes/Yes S3
I4
ASM
AIM
ASM
AIM
336 4
ML6A10
[DW986342]
1, 2, 3 No homology/no recognised domains 13/12 Yes/Yes S2
I3
ASM
AIM
ASM
AIM
320 5
ML8B1
[DW986463]
1, 2, 3 No homology/Gln rich domains 13/11 Yes/Yes S2
I3
ASM
AIM
ASM
AIM
195 3
P0011O14_517
[GT274178]
3 No homology/no recognised domains 18/≥16 No/Yes -
I5
-
AIM
-
AIM
189 3
6G3
[GD272908]
2 No homology/ependymin domain 21/21 Yes/Yes S5
I6
ASM
AIM
ASM
AIM
173 3
ML3E9
[DW986256]
1,2, 3 Perlwapin/3 WAP domains -/≥15 No/No -
-
-
-
ASM
AIM
148 3
ML5B8
[DW986296]
1, 2 No homology/no recognised domains 32/21 Yes/Yes S6
-
-
-
ASM 125 2
ML5H8
[DW986339]
1, 2, 3 No homology/Chitin-Binding domain -/10 Yes/Yes -
-
-
-
ASM
AIM
116 3
  1. *EST libraries: 1 = H. asinina juvenile mantle SMART cDNA library [8]; 2 = H. asinina developmental microarray library [9]; 3 = H. asinina adulte mantle cDNA library [10]. "Pr + Nc" indicates "Prisms + nacre" extract. "≥" indicates that the theoretical molecular mass is at least the following value, as some sequence information is missing.