Table 1 Details of overexpressed proteins identified by MALDI-TOF mass spectrometry in SM resistant M. tuberculosis clinical isolates
Spot No.†| Accession No. | Proteins identified | Mascot score | Nominal mass (Da) | pI | No. of peptides matched | Sequence coverage (%) | ORF No. | Densitometric ratio of protein expression between sensitive vs. resistant isolates |
|---|---|---|---|---|---|---|---|---|---|
1. | P0A5B9 (DNAK_MYCTU) | Chaperone protein dnaK | 165 | 66790 | 4.85 | 22 | 38 | Rv0350 | 1: 1.48 |
2. | P0A520 (CH602_MYCTU) | 60 kDa chaperonin 2 | 85 | 56692 | 4.85 | 12 | 27 | Rv0440 | 1: 3.17 |
3. | P0A5J6 (MDH_MYCTU) | Malate dehydrogenase | 103 | 34358 | 4.65 | 11 | 51 | Rv1240 | 1: 2.45 |
4. | P95083 (P95083_MYCTU) | Hypothetical protein | 98 | 33194 | 4.73 | 9 | 28 | Rv3075c | 1: 3.69 |
5. | P95124 (Y2971_MYCTU) | Probable oxidoreductase | 97 | 30516 | 4.70 | 11 | 47 | Rv2971 | 1: 3.10 |
6. | O53275 (ETFA_MYCTU) | Electron transfer flavoprotein subunit alpha (α-ETF) | 118 | 31672 | 4.71 | 11 | 42 | Rv3028c | 1: 2.97 |
7. | P0A5N2 (AG84_MYCTU) | Antigen 84 | 132 | 28260 | 4.80 | 9 | 44 | Rv2145c | 1: 4.27 |
8. | P0A5B7 (ACR_MYCTU) | 14 kDa Antigen (16 kDa Antigen, HSP16.3) | 117 | 16217 | 5.00 | 9 | 70 | Rv2031c | 1: 2.69 |
9. | O53766 (Y0569_MYCTU) | Hypothetical protein | 80 | 9517 | 5.70 | 5 | 54 | Rv0569 | 1: 2.43 |