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Table 2 Tooth matrix phosphoproteins

From: Phosphoproteomes of Strongylocentrotus purpuratus shell and tooth matrix: identification of a major acidic sea urchin tooth phosphoprotein, phosphodontin

Glean3_entry

Protein

Peptide

No. of P

Best Motif

Cleavage

Tot. no.5

001641a,b

Similar to SM-30

(Sp-Clect)

R. 204LSEPGFFS FLR215 .E (S2.1)

1

-

KR

4

02353 2

Similar to nucleobindin 2a/b

K. 149DGLS AGLPALK159 .E (S2.2)

1

-

KR

1

03345 2

Dickkopf-1 (aa106-339 of A1XR81_STRPU; Sp-Dkk1))

K. 172NDGDLLGYLS AES GEK187 .L (S2.3)

2

CK1

KR

8

05419 2,4

Src family tyrosine kinase (B6DS93; Sp-SFK3)

R. 357IIEDEY IAR365 .E (Table 1)

1

EGFR

KR

1

10288

Similar to pecanex-like protein 1; domain: partial Transmembrane protein 26

(Sp-Tmem26_3)

R. VLGTGT LDR .Q (S2.4)

1

-

KDR

4

105891a

Hypothetical protein; pI 4.1, Gly-rich, Asp-rich (Sp-Hypp_1925)

K. 245PIYIPVS VPR254 .G (S2.5)

1 3

-

KR

KDR

8

11

137631a

Similar to Family with sequence similarity 20, member C/Dentin matrix protein 4; domain: DUF1193

(Sp-Fam20c_1)

K. 407EAMQPYGS DLDDDDFDF -- (S2.6)

1

-

KR

4

14308

Hypothetical protein; domains: EFh; similarity to reticulocalbin

(Sp-Hypp_112)

K. 194VVILDS LEDFDTNK207 .D (S2.7)

1

-

KR

4

14805

Hypothetical protein; domain: EFh; similarity to multiple coagulation factor deficiency protein 2

K. 117DMS VNQIADAR128 .V (S2.8)

1

PLK1

KR

4

151251a

Hypothetical protein: domain: galactose-3-O-sulfotransferase (Sp-Gal3st1_6)

K. 87EDVGGDEES NLAS LEGDK104 .D (S2.9)

2

GSK3, CK1

KR

KDR

2

1

159061a

Hypothetical protein (Sp-Hypp_120)

K. 1237LSVPQGS PLLSR1248 .C (S2.10)

1

GSK3

KR

1

175881a

Hypothetical protein; Val-, Gly-, Pro-, and Ala-rich regions; domains: 2 KAZAL

(Sp-Hypp_136)

K. 316GVAGGS EDVGK327 .G (S2.11)

1

-

KR

4

175901a

222781a

Hypothetical protein/Similar to serotonin receptor 2B; Val-, Gly-, Pro-, and Ala-rich regions; domains: 1 KAZAL

(Sp-Hypp_2346)

R. 492APSPAAPS APR502 .A (S2.12)

R. 392APSAPVRPYAPAPAPQAPSGPSDS S EK418 .Q

(S2.13)

1 3

1

-

CK1 (SS)

KR

KDR

KR

KDR

3

4

19

13

189191a

Hypothetical protein/similar to RPGR/

phosphodontin;

pI 3.9

K. 52(63)EMSS GQVEEPK62(73).E (S2.14)

1 3

-

KR

KDR

3

3

 

(Sp-Hypp_2410)

K. 74EMS S GEGEEQPK85 .E (S2.15)

1

CK2 (S S)

KR

3

  

K. 97(108,119,130,141,152,163,174)EISS GEGEQPK107(118,129,140,151,162,173,184).E (S2.16)

1 3

CK2 (S S)

KDR

2

  

K. 185(229)EISS GEEEQPNEISS GEGEEPK206(250) .E

(S2.17)

2

CK2 (S S)

KR

KDR

10

13

  

K. 218(285)EIS S GEEEQPK228(295) .E (S2.18)

1 3

CK2

KR

KDR

6

4

  

K. 251(296,307)EIS S GEGEEPK261(306,317) .E (S2.19)

1 3

CK2 (S S)

KR

KDR

4

4

  

K. 262(318)EMSS GQVEEQPK273(329).E (S2.20)

1

-

KR

KDR

8

2

  

K. 274(331,341)EMSS GEGYQPK284(340,351) .E (S2.21)

1

CK2 (S S)

KR

3

  

K. 296EIS S GEGEEPKEIS S GEGEEPK317 .E (S2.22)

2

CK2 (S S)

KR

KDR

6

1

  

K. 363EVSS GEGEQPK373 .E (S2.23)

1

CK2 (S S)

KR

4

  

K. 374EVSS GQVEELK384 .G (S2.24)

1 3

CK2 (S S)

KR

KDR

42

8

  

K. 385GMSS GEQEEPK395 .E (S2.25)

1

CK2 (S S)

KR

KDR

3

1

  

K. 396EMSS GEEEQPK406 .E (S2.26)

1

CK2

KR

4

  

K. 407EMS S GEEEEPK417 .E (S2.27)

1

CK2

KR

5

24991 4

Hypothetical protein

R. 142GPS LFGR148 .I (see Table 1 and Additional File 1)

1

PKA/

AKT

KR

KDR

13

19

  1. 1a,b, protein identified previously in test or spine matrix (1a) [10] or tooth matrix (1b) [11]. 2, known phosphoprotein in sea urchin or other eukaryotic species or similar to a known phosphoprotein. 3, non-phosphorylated peptide(s) also identified in the present or previous [10, 11] studies. 4, also in tooth matrix phosphoproteome (Table 2). 5, total number of this phosphopeptide with PEP < 0.01. KR, cleavage with lysyl endopeptidase and trypsin; KD, cleavage with lysyl endopeptidase and endoproteinase Asp-N; KRD, successive cleavage with lysyl endopeptidase, trypsin and endoprotease Asp-N. Phosphorylation sites with a localization probability ≥ 0.75 [39] are printed in bold and underlined. Phosphorylation sites with localization probability 0.5-0.75 are underlined. The entries are ordered according to increasing entry number. For scores and other relevant information see Additional file 1 (Additional file 1: Test matrix protein phosphorylation sites). Annotated best identification spectra are shown in Additional file 2 (Additional file 2: Selected spectra of test matrix phosphopeptides); numbering in brackets is the same as in Additional File 2. SpBase http://sugp.caltech.edu/SpBase/ annotations are given in brackets.
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