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Table 2 The small, acid soluble spore proteins detected in Bacillus subtilis Δ prpE strain.

From: Proteomic analysis of small acid soluble proteins in the spore core of Bacillus subtilis ΔprpE and 168 strains with predictions of peptides liquid chromatography retention times as an additional tool in protein identification

Δprp E

Protein

Peptide sequence

m/z

Charge

X corr

Missed cleavages

c log P

log Sum(k+1) AA

t R exp

t R pred

Δt R

Proteins correctly identified according to X corr values and correctly according to Δt R :

SASP - alpha-type

LVSFAQQNMGGGQF

742.8313

2

4.72

0

-0.78

1.5218

28.39

30.85

-2.46

SASP - alpha-type

RLVSFAQQNMGGGQF

820.9245

2

3.69

1

-1.13

1.5346

30.98

30.99

-0.01

SASP - alpha-type

ANNNSGNSNNLLVPGAAQAIDQMK

814.8842

3

3.41

0

-1.15

1.5762

31.02

32.32

-1.30

SASP - alpha-type

ANGSVGGEITKRLVSFAQQNMGGGQF

1327.9721

2

3.84

2

-0.68

1.696

39.28

36.53

2.75

SASP - beta-type S

ANQNSSNDLLVPGAAQAIDQMK

1143.7547

2

3.25

0

-1.18

1.5526

31.64

31.54

0.10

SASP - beta-type S

LEIASEFGVNLGADTTSR

941.0171

2

4.30

0

-1.25

1.5661

33.14

31.92

1.22

SASP - B. subtilis

NVIQGALEDAGSALKDDPLQEAVQK

1305.9299

2

4.23

1

-1.01

1.628

37.99

34.09

3.90

SASP - B. subtilis

NVIQGALEDAGSALKDDPLQEAVQK

870.9532

3

3.80

1

-1.01

1.628

37.95

34.09

3.86

Proteins incorrectly identified according to X corr values and incorrectly according to Δt R :

SASP - alpha/beta-type

GRRRGV

700.8170

1

0.69

3

-2.48

0.8503

91.69

7.95

83.74

SASP - alpha/beta-type

EQMKLEIAS

525.1123

2

0.85

1

-3.7

1.2459

26.26

19.75

6.51

SASP - gamma-type

AQQVR

601.6790

1

1.22

0

-1.95

0.7843

11.89

6.23

5.66

SASP - gamma-type

EFASE

582.5829

1

0.65

0

-2.58

1.0583

22.38

14.57

7.81

SASP - gamma-type

QNQQSAGQQGQFGTEFASETDAQQVR

1421.4511

2

1.90

0

-1.72

1.6016

24.73

32.70

-7.97

SASP - gamma-type

QQSAAGQGQFGTEFASETNAQQVRKQNQ

1014.0562

3

1.64

2

-1.43

1.6228

27.28

33.60

-6.32

SASP - gamma-type

KQNQQSAAGQGQFGTEFASETNAQQVRK

1014.0705

3

2.36

2

-1.27

1.6228

27.37

33.73

-6.36

SASP - B. subtilis

ALKDDPLQEAVQKKKNNR

1048.6888

2

0.78

4

-1.49

1.4324

37.98

27.43

10.55

SASP SspI

PGLGVLFEV

931.1109

1

0.78

0

0.81

1.408

3.28

28.40

-25.12

SASP Tlp

QNGYR

637.6682

1

0.54

0

-2.24

0.9333

91.35

10.80

80.55

Potential false-positives: proteins correctly identified according to X corr values and incorrectly according to Δt R :

SASP - gamma-type

KQNQQSAGQQGQFGTEFASETDAQQVR

990.6917

3

6.97

1

-1.43

1.6123

23.22

33.27

-10.05

SASP - gamma-type

QQNQSAEQNKQQNS

816.8161

2

2.81

1

-2.74

1.1461

9.12

17.27

-8.15

SASP - gamma-type

KQNQQSAAGQGQFGTEFASETNAQQVR

971.3462

3

5.20

1

-1.33

1.6123

25.79

33.34

-7.55

SASP - gamma-type

KQNQQSAAGQGQFGTEFASETNAQQVR

1456.5194

2

5.75

1

-1.33

1.6123

25.84

33.34

-7.50

SASP - B. subtilis

VVVSVNTDQDQAQAQSQDGED

1117.6176

2

4.73

0

-2.47

1.4038

19.36

25.77

-6.41

Potential false-negatives: proteins incorrectly identified according to X corr values and correctly according to Δt R :

SASP - beta-type S

ANGSVGGEITKR

595.1525

2

1.82

1

-1.75

1.2151

22.14

20.24

1.90

SASP - B. subtilis

NVIQGALEDAGSALKDDPLQEAVQKK

913.6775

3

2.36

2

-0.88

1.6381

38.31

34.52

3.79

SASP - B. subtilis

LTGGVTPQGDLEGNTHNDPKTELEER

936.9845

3

2.69

1

-1.59

1.5917

27.91

32.48

-4.57

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ISSN: 1477-5956