Table 3 The small acid soluble spore proteins detected in Bacillus subtilis 168 strain.
168 | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
Protein | Peptide sequence | m/z | Charge | X corr | Missed cleavages | c log P | log Sum(k+1) AA | t R exp | t R pred | Δt R |
Proteins correctly identified according to X corr values and Δt R : | ||||||||||
SASP - alpha-type | LVSFAQQNMGGGQF | 742.8313 | 2 | 4.73 | 0 | -0.78 | 1.5218 | 28.61 | 30.85 | -2.24 |
SASP - beta-type S | ANGSVGGEITK | 517.0593 | 2 | 2.40 | 0 | -1.58 | 1.1878 | 16.22 | 19.49 | -3.27 |
Proteins incorrectly identified according to X corr values and Δt R : | ||||||||||
SASP - alpha/beta-type | LEIASEFGVQLGAETTSR | 637.0292 | 3 | 0.54 | 0 | -1.08 | 1.5661 | 19.25 | 32.05 | -12.80 |
SASP - alpha/beta-type | DLGFYDTVK | 1058.1664 | 1 | 0.79 | 0 | -2.79 | 1.3818 | 96.39 | 24.82 | 71.57 |
SASP - beta-type S | LVSFAQQQMGGR | 1322.5195 | 1 | 0.65 | 0 | -1.39 | 1.3947 | 81.37 | 26.30 | 55.07 |
SASP - beta-type S | LEIASEFGVNLGADTTSR | 941.0171 | 2 | 2.07 | 0 | -1.25 | 1.5661 | 40.37 | 31.92 | 8.45 |
SASP - gamma-type | QQNQSAEQNK | 588.0960 | 2 | 0.84 | 0 | -1.72 | 1 | 5.64 | 13.34 | -7.70 |
Potential false-negatives: proteins incorrectly identified according to X corr values and correctly according to Δt R : | ||||||||||
SASP - B. subtilis | DAAVAK | 574.6503 | 1 | 0.92 | 0 | -2.75 | 0.8503 | 9.64 | 7.74 | 1.90 |