Use of cervicovaginal fluid for the identification of biomarkers for pathologies of the female genital tract

Table 1 Overview of primarily qualitative proteomic studies performed on human CVF and cervical mucus.

*Study*	*Samples*	*Separation method*	*MS method*
Venkataraman et al., 2005[38]	Undiluted CVF collected in cup from healthy women (postmenarcheal, pre-menopausal)	2D-PAGE (1D: AU-PAGE; 2D: Tricine- SDS-PAGE)	MALDI-TOF-TOF
Di Quinzio et al., 2007[40]	Swabs from pregnant women (37 weeks gestation)	2D-PAGE (1^st D: IEF; 2^nd D SDS-PAGE) followed by RP-LC	MALDI-TOF or ESI-linear IT
Dasari et al., 2007[42]	Swabs from pregnant women (18,5 weeks gestation as mean)	1D-SDS-PAGE followed by offline 2D(SCX/RP)-LC	ESI-Q-TOF
Tang et al., 2007[45]	Washings from clinically normal women; 7 washings from women infected with Candida spp.	2D-PAGE (1^st D: IEF; 2^nd D SDS-PAGE)	MALDI-TOF-TOF
Shaw et al., 2007[44]	Gauze from healthy women	1D-SDS-PAGE or SCX-LC both followed by RP-LC	ESI-linear IT
Pereira et al., 2007[43]	Swabs from pregnant women (15.8-35.9 weeks gestation)	2D-DIGE or MudPIT(SCX/RP)-LC	ESI-Q-TOF
Andersch-Björkman et al., 2007[36]	Cervical aspiration using syringe from healthy women	1D-PAGE or SDS-agarose electrophoresis followed by RP-LC	ESI-FT-ICR
Klein et al., 2008[14]	Swabs from pregnant women (30.5 weeks gestation as mean)	RP-LC	ESI-IT
Zegels et al., 2009[22]	Washings from HPV-infected women	Ultrafiltration or C₄-LC protein fractionation/C₁₈-LC peptide separation	MALDI-TOF-TOF
Panicker et al., 2010[37]	Cervical mucus from healthy women obtained with sponges	2D-PAGE (1^st D: IEF; 2^nd D SDS-PAGE) or 1D-PAGE followed by RP-LC	ESI-Q-TOF

For each study the following information is presented: 1) the nature of the samples which were used in the study (sample collection method and patient physiology), 2) the methods used to separate proteins and peptides and 3) MS method used for analysis.

ISSN: 1477-5956