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Table 3 Vesicular trafficking-related proteins identified by dMS and SC.

From: A label-free differential quantitative mass spectrometry method for the characterization and identification of protein changes during citrus fruit development

    

Stage II (55 mm) vs. early stage II (35 mm)

Stage III (80 mm) vs. Stage II (55 mm)

    

dMS

SC

dMS

SC

Gene Family

Annotation

iCitrus ID

Blast Hit TAIR ID

Peptides

Ratio

Bayes

Factor

Fold Change

Direction*

Peptides

Ratio

Bayes

Factor

Fold Change

Direction*

Rab

RABA1a/ARA2

5282

AT1G06400

3

0.03

--

--

--

2

2.85

--

--

--

 

RABA1d/Rab11B

50939

AT4G18800

7

0.025

1.34

1.52

0

--

--

0.95

1.53

0

 

RABA1f

23943

AT5G60860

5

0.11

465

31.9

-1

--

--

0.8

1.2

0

 

RABA2a/Rab11C

33548

AT1G09630

6

0.72

63362

23

-1

2

7.43

31.8

4.5

1

 

RABA2b

27900

AT1G07410

3

0.026

3302

28

-1

--

--

--

--

--

 

RABB1b/Rab2C

28361

AT4G35860

--

--

--

--

--

3

33.41

--

--

--

 

RABB1c

57271

AT4G17170

5

0.11

72

3.2

-1

4

28.6

1555

5.02

1

 

RABD1/FP8

44137

AT3G11730

3

0.33

395

17.8

-1

--

 

0.9

1.45

0

 

RABD2a/Rab1b

22194

AT1G02130

3

0.07

1472

24

-1

2

31.24

62.5

5.00

1

 

RABD2b/Rab1A

21238

AT5G47200

2

0.04

--

--

--

--

--

--

--

--

 

RABE1a/Rab8

55887

AT3G53610

3

0.03

54.9

3.6

-1

--

--

1.04

1.5

0

 

RABE1e/Rab8E

58806

AT3G09900

2

0.04

--

--

--

--

--

--

--

--

 

RABE1c/Rab8/ARA-3

21701

AT3G46060

4

1.06

8.8

1.8

0

2

3.48

0.82

1.2

0

 

RABG3d

44916

AT1G52280

2

0.01

--

--

--

--

--

--

--

--

 

RABG3f/Rab7B

30351

AT3G18820

3

0.006

0.8

1.3

0

2

6.94

2.16

1.79

0

 

RABH1b/Rab6A

53105

AT2G44610

5

0.07

478

18.3

-1

--

--

0.75

1.2

0

 

RABH1e

30604

AT5G10260

2

0.05

--

--

--

--

--

--

--

--

Arf

ARLA1c

26509

AT3G49870

3

0.79

0.58

1.13

0

2

4.49

1.07

1.67

0

 

ARFA1e

422

AT3G62290

3

0.13

--

--

--

3

26.62

--

--

--

 

ARFA1f

22081

AT1G10630

5

0.15

--

--

--

6

19.71

--

--

--

 

SAR1c

34375

AT4G02080

--

--

--

--

--

3

4.91

--

--

--

Ran

STL2P/SEC12P-Like

54385

AT2G01470

--

--

2319

5.37

1

--

--

0.67

1.04

0

 

RANBP1

42600

AT5G58590

5

0.5

3.4

2.8

0

7

129.80

0.6

1.98

0

 

RANBP1b

2905

AT2G30060

--

--

1.3

4.2

0

5

130.08

280

22.3

1

 

RAN3

57970

AT5G55190

2

0.87

0.64

1.82

0

7

20.25

3.7

1.67

0

Rho

GP3/ROP4

29311

AT1G75840

2

0.28

--

--

0

--

--

--

--

--

GDI

GDI1

34016

AT2G44100

--

--

1.58

2.18

0

3

5.00

0.73

1.13

0

 

GDI2-like

876

AT5G09550

--

--

--

--

--

2

3.11

--

--

--

VAMP/R-SNAREs

SEC22

58654

AT1G11890

2

0.20

26.9

13.6

-1

--

--

1.06

1.46

0

 

VAP27-1

54676

AT3G60600

2

0.77

--

--

--

--

--

--

--

--

 

VAMP713

23669

AT5G11150

2

1.41

--

--

--

--

--

--

--

--

Qa-SNAREs

SYP132 (syntaxin 132)

12539

AT5G08080

--

--

3

6.2

0

2

3.21

1.25

1.65

0

 

VAM3 (syntaxin 22)

37248

AT5G46860

--

--

1.35

1.86

0

--

--

4.5

2.3

0

Qb-SNAREs

VTI11

24253

AT5G39510

2

0.05

0.65

1.37

0

--

--

0.5

1.2

0

Qc-SNAREs

SYP71 (SYNTAXIN)

27696

AT3G09740

--

--

2.17

4.34

0

--

--

--

--

--

 

ALPHA-SNAP2

2375

AT3G56190

--

--

1.00

1.00

0

--

--

6.41

7.6

0

ESCRT III

SNF7.1

35782

AT4G29160

4

1.31

4.88

2.99

0

7

597.36

106

5.5

1

Dynamin

ADL6 (dynamin-like protein 6)

36589

AT1G10290

--

--

--

--

--

2

6.63

--

--

--

others

SEC61

35474

AT1G29310

3

0.10

171.70

17.65

-1

--

--

1.00

1.4

0

 

SEC14

30056

AT1G72160

5

3.42

37.17

13.65

1

16

12.92

4.8

1.5

0

 

PATL2

35474

AT1G22530

3

0.104

170.5

17.65

-1

3

6.83

1.04

1.43

0

 

SCAMP

34675

AT1G32050

3

1.70

1.50

1.55

0

2

4.11

0.74

1.18

0

 

SC3 (secretory carrier 3)

30772

AT1G61250

2

0.98

2.9

6.12

0

--

--

1.00

1.02

0

 

SYT1

45523

AT2G20990

4

8.7

0.3

1.5

0

4

2.64

1.2

1.6

0

 

COP-I coatomer, B-COPα

29871

AT2G21390

2

0.4

0.6

1.22

0

3

11.47

1.31

1.73

0

 

RTNLB3 reticulon

42672

AT1G64090

2

0.53

2.1

3.7

0

2

2.31

0.72

1.48

0

 

RTNLB5 reticulon

13580

AT2G46170

--

--

0.97

1.02

0

--

--

4.35

2.97

0

 

RTNLB6 reticulon

22293

AT3G10260

--

--

1.24

1.4

0

2

36.56

187.81

5.76

1

 

clathrin heavy chain

57153

AT3G08530

--

--

83.92

13.36

-1

--

--

0.76

1.17

0

 

clathrin heavy chain

60690

AT3G08530

2

0.05

63.7

13.65

-1

--

--

46.45

10.98

-1

 

Clathrin light chain

51128

AT2G20760

2

119

2300591

11.55

1

2

1.8

0.8

1.2

0

 

Clathrin light chain

21278

AT3G51890

2

118

1511

6.2

1

--

--

1.63

1.57

0

 

myosin heavy chain

38388

AT4G31340

--

--

0.6

1.00

0

--

--

0.75

1.3

0

 

myosin heavy chain

37311

AT1G06530

3

0.02

156

16

-1

--

--

1.1

1.4

0

  1. * The column "direction" under spectral counting measurement represent expression direction, 1 = up-regulated, 0 = no change, -1 = down-regulated.
  2. Proteins identified by dMS were considered to be upregulated when expression fold > 2, not changed when 0.5 < fold < 2 and down-regulated when fold change was < 2. For SC Bayes factor of > 10 was used for significance difference.