Figure 7
From: RNAi-based validation of antibodies for reverse phase protein arrays
Determination of isoform-specificity by RNAi and its application for network analysis. AKT 1 and AKT 2 as well as cyclin family members CCND1 and CCND3 were knocked down individually and the protein levels were then detected specifically by RPPA. Low protein levels are represented in blue and high levels in red (A). The 27 indicated genes were knocked down individually with specific siRNAs and the effect on AKT1 protein levels was measured using an isoform-specific antibody. The heatmap section shows a reduced (blue) or elevated expression (red) of AKT1 in response to a targeted knockdown of the 27 genes (B).