Figure 8
From: RNAi-based validation of antibodies for reverse phase protein arrays
Knockdown validation using quantitative real time PCR. Knockdown of AKT1 and AKT2 as well as CCND1 and CCND1 mRNAs upon siRNA transfection into MDA-MB-231 cells was measured by quantitative real-time PCR. Respective siRNAs applied in the transfection are indicated in relation to the relative mRNA expression levels (values normalized to non-targeting controls ACTB and HPRT mRNAs).