Table 2 Quantitative proteomic techniques that have been applied to clinical proteomics using labeling-free method
From: Clinical proteomics for liver disease: a promising approach for discovery of novel biomarkers
Methods | Types of chips or magnetic beads | Interests | References |
|---|---|---|---|
ProteinChip SELDI | IMAC30 (metal modified), CM10 (cation exchanging), WCX2 (weak cation exchanging) Q10 (anion exchanging), H50 (reverse-phase), H4 (reverse-phase), NP20 (normal-phase), Gold | Analyses: a few μl of serum/plasma (without removal of abundant proteins), urine, cell/tissue lysates and conditioned media Identification: MS/MS, LC-MS/MS | [21] |
ClinProt® | Profiling: WCX, WAX, HIC8, IMAC-Cu Large-protein beads: HIC1, HIC3 Peptides beads: HIC18 Phospho beads: IMAC-Fe Glyco beads: LAC ConA, ConAC boronic Antibody capture beads: IAC ProtG | Performance: Many samples can be assayed in parallel. | [22] |