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Figure 1 | Proteome Science

Figure 1

From: Improving the yeast two-hybrid system with permutated fusions proteins: the Varicella Zoster Virus interactome

Figure 1

Vectors described in this study including their parental vectors. pGBKT7g and pGADT7g generate N-terminal fusions of DNA-binding (DBD) and activation domain (AD) fusions, respectively. The new vectors pGBKCg and pGADCg fuse DBD and AD at the C-terminus of inserted ORFs. Note that both pGBK vectors use a truncated version of the ADH promoter (indicated by *) which may reduce expression levels and thus interaction signals [26, 27].

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