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Table 5 Proteins with top ten highest sequence coverage from tissue lysis protocol and cell suspension protocol.

From: A novel method for sample preparation of fresh lung cancer tissue for proteomics analysis by tumor cell enrichment and removal of blood contaminants

Enriched Tumor cell Suspension samples (ETS)

%Coverage

Accession

Name

45

IPI00479145.2

KRT19 Keratin, type I cytoskeletal 19

45

IPI00719280.2

UBB;RPS27A;UBC ubiquitin B precursor

40

IPI00894498.1

ACTB Beta actin variant (Fragment)

34

IPI00795719.1

cDNA FLJ53570, highly similar to Keratin, type I cytoskeletal 16

34

IPI00641249.2

ATP5A1 18 kDa protein

32

IPI00789324.3

JUP cDNA FLJ60424, highly similar to Junction plakoglobin

32

IPI00396378.3

HNRNPA2B1 Isoform B1 of Heterogeneous nuclear ribonucleoproteins A2/B1

31

IPI00554648.3

KRT8 Keratin, type II cytoskeletal 8

30

IPI00747707.2

KRT17 Radiated keratinocyte mRNA 266

29

IPI00216952.1

LMNA Isoform C of Lamin-A/C

Fresh-Frozen samples (FF)

%Coverage

Accession

Name

39

IPI00790892.1

ENO2 6 kDa protein

36

IPI00796636.14

HBB Hemoglobin (Fragment)

28

IPI00027462.1

S100A9 Protein S100-A9

27

IPI00217473.5

HBZ Hemoglobin subunit zeta

24

IPI00745872.2

ALB Isoform 1 of Serum albumin

22

IPI00910407.1

cDNA FLJ53060, moderately similar to Peptidyl-prolyl cis-trans isomerase A

22

IPI00030929.4

MYL9 myosin regulatory light chain 9 isoform b

21

IPI00894498.1

ACTB Beta actin variant (Fragment)

21

IPI00903243.1

LOC284100 cDNA FLJ37577 fis, clone BRCOC2003513

20

IPI00747707.2

KRT17 Radiated keratinocyte mRNA 266