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Table 8 Identification and quantification of farinins, purinins, triticins, globulins, and grain softness proteins by 2DE-MS/MS.

From: Deciphering the complexities of the wheat flour proteome using quantitative two-dimensional electrophoresis, three proteases and tandem mass spectrometry

Spot # Predominant protein1 Total
proteins
in spot2
Spot
Volume3
Std
Dev4
MS/MS
Coverage5,8
Predicted
MW6,8
pI7,8
Farinins       
196 Farinin Bu-1 full length 1 0.04 0.007 15 29978 8.1
385 Farinin Bu-1 C-terminus 1 0.07 0.013 13 18832 8.4
386 Farinin Bu-1 C-terminus 1 0.14 0.030 24 18832 8.4
193 Farinin Bu-2 2 0.28 0.052 38 30567 7.5
207 Farinin Bu-2 1 0.04 0.037 25 30567 7.5
549 Farinin Bu-2 1 <0.01 0.000 20 30567 7.5
336 Farinin Bu-3 2 0.33 0.043 45 30883 7.9
345 Farinin Bu-3 3 <0.01 0.000 34 30883 7.9
Purinins       
542 Purinin Bu-1 3 0.07 0.007 24 20272 5.9
543 Purinin Bu-1 2 0.22 0.015 38 20272 5.9
219 Purinin Bu-2 1 0.06 0.015 34 20592 6.2
223 Purinin Bu-2 1 0.20 0.030 34 20592 6.2
220 Purinin Bu-3 1 0.23 0.018 19 22371 6.2
227 Purinin Bu-3 1 0.04 0.005 28 22371 6.2
Triticins (Tri-1)       
143 Triticin [GenBank:DR736644] N-terminal subunit 3 0.27 0.011 inc inc inc
136 Triticin TC11_285558, N-terminal subunit 2 0.17 0.011 36 40501 6.2
348 Triticin TC11_285558, N-terminal subunit 2 0.23 0.023 36 40501 6.2
423 Triticin TC11_285558, N-terminal subunit 1 0.46 0.059 36 40501 6.2
463 Triticin TC11_285558, N-terminal subunit 1 0.06 0.003 18 40501 6.2
249 Triticin TC11_264477, C-terminal subunit 1 0.21 0.011 22 21830 8.1
253 Triticin TC11_264477, C-terminal subunit 1 0.19 0.010 17 21830 8.1
Globulins       
218 Globulin-1 [GenBank:ABG68030] (Glo-2) 1 0.05 0.023 48 22941 8.6
104 Globulin-2 Bu-17295 1 0.02 0.004 25 53832 6.6
121 Globulin-2 Bu-17295 1 0.04 0.002 23 53832 6.6
99 Globulin-2 Bu-17366 1 0.03 0.002 inc inc 7.0
103 Globulin-2 Bu-18428 1 0.06 0.003 10 53554 6.6
106 Globulin-2 Bu-18428 1 0.03 0.004 30 53554 6.6
180 Globulin Glo-3-type TC234094 5 0.08 0.007 inc inc inc
184 Globulin Glo-3-type TC11_305389 2 0.03 0.008 inc inc inc
309 Globulin Glo-3-type TC11_ 305389 1 0.02 0.004 inc inc inc
2729 Globulin Glo-3-type TC234094/WTAI-CM3 [SwissProt: P17314] 2 0.02 0.003 nd nd nd
GSP and Puroindoline (Pin-D1)       
174 Grain softness protein [GenBank:CAA56591] 1 0.02 .001 17 16157 8.1
275 Grain softness protein [GenBank:CAA56586] 2 0.06 0.009 30 16381 7.6
248 Puroindoline-b [GenBank:AAT40244] 2 0.21 0.053 28 14812 9.0
271 Puroindoline-b [GenBank:AAT40244] 1 0.04 0.015 44 14812 9.0
  1. Loci are in parentheses.
  2. 1-7 Footnotes as in Table 4.
  3. 8inc indicates sequence was incomplete. nd indicates not determined because no single protein had a predominant number of peptides.
  4. 9Spot contained a similar number of peptides from two different proteins. Details are in Additional file 1.