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Table 2 List of differentially expressed protein spots in kidney tissues identified by MALDI-TOF-TOF MS and MS/MS analysis

From: Proteomic analysis of chicken embryonic trachea and kidney tissues after infection in ovo by avian infectious bronchitis coronavirus

Spota

Accession Numberb

Protein Description

Mr(KDa)/ p I

Score

Coverage (%)d

Normalized spot volume (vol%)e

p

Ratio (infected/mock-infected)

Protein function

Other viruses found inf

      

Mock-infected

IBV-infected

    

Cytoskeletal proteins

2

gi|515694

Tropomyosin beta chain [Gallus gallus]

28.5/4.65

123

31

0.2134 ± 0.0092

0.0524 ± 0.0162

< 0.001

0.25

Actin binding

HBV, HCV, WSSV

3

gi|45382323

Tropomyosin 1 alpha [Gallus gallus]

32.9/4.73

307

57

0.1907 ± 0.0219

0.0474 ± 0.0188

0.001

0.25

Actin binding

PRRSV, RSV, HPV8, VHSV, CVB3

Calcium ion-binding proteins

1

gi|45382533

annexin A2 [Gallus gallus]

38.7/6.92

389

67

0.0388 ± 0.0177

0.2153 ± 0.0838

0.023

5.55

Phospholipase inhibitor activity. Calcium ion binding

CSFV, PRRSV, HBV, HIV, DHBV, WNV

12

gi|45382533

annexin A2 [Gallus gallus]

38.6/6.92

505

69

0.5186 ± 0.1260

0.1549 ± 0.0474

0.009

0.3

Phospholipase inhibitor activity. Calcium ion binding

 

4

gi|71895873

annexin 5 [Gallus gallus]

36.2/5.6

402

71

0.4276 ± 0.0516

0.1898 ± 0.0334

0.003

0.44

Calcium ion binding. Calcium-dependent phospholipid binding

PRRSV, DV, DHBV, HBV, VHSV

17

gi|50982399

annexin A6 [Gallus gallus]

75.2/5.57

441

49

0.4317 ± 0.0389

0.1653 ± 0.0107

< 0.001

0.38

Calcium ion binding. Calcium-dependent phospholipid binding

 

19

gi|45382893

calbindin 1, 28 kDa [Gallus gallus]

30.4/4.72

212

60

N/A

0.1485 ± 0.0614

0.014

N/A

Calcium ion binding. Vitamin D binding

 

20-1

gi|45382893

calbindin 1, 28 kDa [Gallus gallus]

30.2/4.72

285

50

N/A

0.1527 ± 0.0545

0.008

N/A

Calcium ion binding. Vitamin D binding

 

Carbohydrate and lipid metabolic proteins

5

gi|46048696

carbonic anhydrase II [Gallus gallus]

29.4/6.56

354

75

0.2622 ± 0.0554

0.4806 ± 0.0850

0.020

1.83

Morphogenesis of an epithelium. One-carbon metabolic process

 

8

gi|71895267

carbonyl reductase 1 [Gallus gallus]

30.5/8.5

357

82

0.1847 ± 0.0160

0.0909 ± 0.0220

0.004

0.49

Catalytic activity. Oxidoreductase activity

VHSV

10

gi|45383766

L-lactate dehydrogenase B [Gallus gallus]

36.3/7.07

306

40

0.3102 ± 0.0260

0.6368 ± 0.1152

0.009

2.05

Glycolysis. Oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor

PRRSV, HBV, HIV, IBDV, REOV

11

gi|118090053

similar to L-3-hydroxyacyl-Coenzyme A dehydrogenase, short chain [Gallus gallus]

34.4/8.68

135

39

0.0814 ± 0.0048

0.2186 ± 0.0820

0.044

2.69

Catalytic activity. Oxidoreductase activity. Fatty acid metabolic process

 

14

gi|118093509

PREDICTED: similar to cytosolic NADP-dependent isocitrate dehydrogenase [Gallus gallus]

46.6/8.02

412

47

0.2077 ± 0.0198

0.0942 ± 0.0092

0.001

0.45

Oxidoreductase activity

PRRSV, PCV2, HBV, RSV

15

gi|46048768

enolase 1 [Gallus gallus]

47.3/6.17

387

57

0.5718 ± 0.1537

1.2489 ± 0.0439

0.002

2.18

Glycolysis

PRRSV, PCV2, WSSV, HSV-1, RSV, DHBV, HIV, IBDV, HBV, VHSV, WNV, SARS-CoV

18

gi|110591367

Chain A, The Structure Of Chicken Mitochondrial Pepck

67.3/6.55

441

49

0.0866 ± 0.0138

0.1704 ± 0.0253

0.007

1.97

Gluconeogenesis

HBV, SARS-CoV

Amino acid metabolic proteins

6

gi|2981970

glutathione S-transferases 2 [Gallus gallus]

25.8/7.0

437

68

0.1877 ± 0.0201

0.4192 ± 0.0767

0.007

2.23

Amino acid metabolic process

HBV

13

gi|118094764

PREDICTED: similar to cystathionase [Gallus gallus]

43.9/6.86

399

35

0.1704 ± 0.0390

0.3164 ± 0.0547

0.02

1.86

Cysteine biosynthetic process

 

Antioxidative stress proteins

7

gi|50751518

PREDICTED: similar to peroxiredoxin-1 [Gallus gallus]

22.3/8.24

252

42

0.5474 ± 0.0371

0.1253 ± 0.0600

< 0.001

0.23

Response to oxidative stress. Removal of superoxide radicals. Regulation of stress-activated MAPK cascade

CSFV, PRRSV, RSV, SARS-CoV, HBV, IAV

Protein processing

16

gi|57530409

CNDP dipeptidase 2 [Gallus gallus]

53.1/5.71

403

52

0.1743 ± 0.0319

0.3196 ± 0.0759

0.038

1.83

Proteolysis

 

9

gi|45382969

sulfotransferase family, cytosolic, 1C, member 3 [Gallus gallus]

36.2/6.68

245

47

0.1481 ± 0.0412

0.2923 ± 0.0578

0.024

1.97

Sulfotransferase activity. Detoxicating

 

Immune response proteins

20-2

gi|54606655

MHC class I antigen [Gallus gallus]

37.6/6.09

385c

71

N/A

0.1527 ± 0.0545

0.008

N/A

Immune response. Antigen processing and presentation

BVDV

  1. a) Spot ID: is the unique number which refers to the labels in Figure 4.
  2. b) Accession Number: gi number in NCBI.
  3. c) Score: Protein score based only on MS spectra by MALDI-TOF, other spots based on combined MS and MS/MS spectra from MALDI-TOF-TOF identification, a protein score greater than 83 is significant in this study (P < 0.05).
  4. d) Sequence coverage (%): Percentage of identified protein sequences covered by matched peptides.
  5. e) Values are presented as mean ± SD. n = 3; vol % was defined as the ratio of the intensity volume of each spot to that of all spots calculated by the software. The SD represents standard deviation of the vol % in three biological replicates.
  6. N/A: A indicates the spot was detectable on one of the gels, N indicates the spot was too weak to detect on one of the gels.
  7. f) HCV, Hepatitis C virus; VSV, Vesicular stomatitis virus; HSV-1, Herpes simplex virus type-1; DV, Dengue virus; HIV, Human immunodeficiency virus; SARS-CoV, Severe acute respiratory syndrome -associated coronavirus; VHSV, Viral haemorrhagic septicemia virus; WNV, West Nile virus; BVDV, Bovine viral diarrhea virus.
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Proteome Science

ISSN: 1477-5956