Figure 9
From: Systems analysis of endothelial cell plasma membrane proteome of rat lung microvasculature
Validation of neuron-restricted proteins at the EC PM. The possibility of the neuron-restricted proteins at the EC PM was investigated by: IHC analysis of human normal lung vasculature from Human Protein Atlas with plexin D1 as positive control (a) and EM of rat lungs stained with anti-contactin 1 (top) or anti-neuropilin 1 (bottom) antibody and gold particles (b) (see Materials and Method). Subcellular locations of mitochondrion (Mit), endothelium (Endo), basement membrane (BM), epithelium (Epi), and plasma membrane (arrowhead) of endothelial cells (ECs) at the luminal surface of blood vessels are as indicated in each image.