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Figure 1 | Proteome Science

Figure 1

From: Stable isotope shifted matrices enable the use of low mass ion precursor scanning for targeted metabolite identification

Figure 1

Multiple precursor ion scanning for GluFib from within a complex mixture. 1 pmol of GluFib was added to 40 μg 15N matrix consisting of the acid soluble fraction of E. coli. A double precursor ion scan was performed using the immonium ion targets of (A) 72 (valine) and (C) 120 (phenylalanine). Figure 1, shows the precursor ion scans both detect the 786 m/z ion (B and D respectively) and subsequently trigger IDA of a product ion spectra (E) that produces sequencing ions for the GluFib peptide EGVNDNEEGFFSAR.

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Proteome Science

ISSN: 1477-5956