Skip to main content

Table 1 Approximate Yield from Each Depletion Method

From: Evaluation of three high abundance protein depletion kits for umbilical cord serum proteomics

Method

Load Volume (μl)

Load (μg)

Yield (μg)

Yield %

Yield Concentration (μg/μl)

Blue Albumin and IgG Depletion Kit (PROTBA, Sigma-Aldrich)

25

1500

216

14.39

1.727

Multiple Affinity Removal System (5185, Agilent)

20

1200

133

11.08

2.871*

Immunoaffinity Albumin and IgG Depletion Kit (PROTIA, Sigma-Aldrich)

50

3000

340

11.34

1.513

  1. *The protein concentration in the depleted solution of Multiple Affinity Removal System (Agilent) was detected after concentration by ultrafiltration using a 5000 Da molecular mass cut-off centrifugal concentrator.
Back to article page

Proteome Science

ISSN: 1477-5956