Comparative proteomics analysis of proteins expressed in the I-1 and I-2 internodes of strawberry stolons

Fang, Xianping; Ma, Huasheng; Lu, Dezhao; Yu, Hong; Lai, Wenguo; Ruan, Songlin

doi:10.1186/1477-5956-9-26

Proteome Science

Table 1 Identified proteins from the strawberry stolon I-1 internode

From: Comparative proteomics analysis of proteins expressed in the I-1 and I-2 internodes of strawberry stolons

Spot No. ^a	Protein	Accession Number ^b	Molecular Function ^c	Reference Organism	Theoretical kDa/pI ^d	Experimental kDa/pI ^e	Score ^f	SC ^g (%)	Matched/Unmatched queries ^h
	Metabolism
15	Malate dehydrogenase	P83373	L-malate dehydrogenase activity	Fragaria ananassa	35.8/8.7	13.5/5.3	99	41	9/43
29	Glucan endo-1,3-beta-glucosidase 6	Q93Z08	glucan endo-1,3-beta-D-glucosidase activity	Arabidopsis thaliana	52.6/5.6	20.3/5.8	58	27	6/35
31	Glucan endo-1,3-beta-glucosidase 6	Q93Z08	glucan endo-1,3-beta-D-glucosidase activity	Arabidopsis thaliana	52.6/5.6	20.4/5.0	63	21	5/42
40	Uricase	O04420	urate oxidase activity	Arabidopsis thaliana	35.0/8.6	23.4/6.1	58	38	10/107
42	GDSL esterase/lipase At4g16220	O23469	hydrolase activity, acting on ester bonds	Arabidopsis thaliana	26.7/8.9	23.2/7.8	57	37	6/36
90	Malate dehydrogenase, mitochondrial	P83373	L-malate dehydrogenase activity	Fragaria ananassa	35.8/8.7	40.3/8.2	122	51	10/50
91	Malate dehydrogenase, mitochondrial	P83373	L-malate dehydrogenase activity	Fragaria ananassa	35.8/8.7	43.3/8.3	100	50	9/45
111	S-adenosylmethionine synthase	Q8W3Y4	metal ion binding	Phaseolus lunatus	43.5/5.6	47.4/5.8	74	24	7/9
118	2,3-bisphosphoglycerate-independent phosphoglycerate mutase	P30792	manganese ion binding	Zea mays	60.7/5.2	41.3/4.9	59	24	8/27
133	Fructokinase-1	A2WXV8	ATP binding	Oryza sativa	34878/5.1	36.1/3.8	76	23	6/15
142	S-adenosylmethionine synthase 4	A9PHC5	metal ion binding	Populus trichocarpa	42999/5.7	46.5/6.3	74	18	5/2
162	Phosphoglucomutase	P93804	magnesium ion binding	Zea mays	63286/5.4	60.2/6.1	63	17	9/30
	Energy
152	ATP synthase subunit beta, chloroplastic	Q9MRR9	ATP binding	Brasenia schreberi	53.8/5.2	45.3/4.8	76	27	8/19
153	ATP synthase subunit beta	Q01859	ATP binding	Oryza sativa	59.0/5.9	48.1/5.4	134	44	19/45
155	ATP synthase subunit beta	Q6QBP2	ATP binding	Castanea sativa	53.8/5.3	45.9/4.4	108	37	15/26
156	ATP synthase subunit alpha	A4QJA0	ATP binding	Aethionema cordifolium	55.3/5.2	58.5/3.5	114	22	10/13
	Photosynthesis
2	Ribulose bisphosphate carboxylase small chain 1A	P10795	monooxygenase activity	Arabidopsis thaliana	20.5/7.6	10.7/7.8	62	43	7/101
37	Ribulose bisphosphate carboxylase large chain (Fragment)	O98681	ribulose-bisphosphate carboxylase activity	Zamioculcas zamiifolia	49.9/6.3	21.4/4.9	79	25	10/78
46	Ribulose bisphosphate carboxylase large chain	P28439	magnesium ion binding	Pelargonium hortorum	53.3/6.3	23.7/7.5	62	32	13/104
69	Triosephosphate isomerase	Q9M4S8	triose-phosphate isomerase activity	Fragaria ananassa	33.7/7.6	30.0/5.1	152	55	19/112
72	Triosephosphate isomerase	Q9M4S8	triose-phosphate isomerase activity	Fragaria ananassa	33.7/7.6	31.3/5.6	126	59	16/77
126	Oxygen-evolving enhancer protein 1	P26320	calcium ion binding	Solanum tuberosum	35.6/5.8	35.6/4.4	111	33	8/8
127	Oxygen-evolving enhancer protein 1	P26320	calcium ion binding	Solanum tuberosum	35.6/5.8	34.9/4.8	113	28	9/21
	Transcription
6	Histone acetyltransferase GCN5	Q9AR19	protein binding	Arabidopsis thaliana	63.5/6.0	14.2/4.1	59	29	11/66
18	Pentatricopeptide repeat-containing protein At3g09650	Q9SF38	nucleotide binding	Arabidopsis thaliana	84.4/6.5	11.2/6.2	80	23	14/49
27	Transcription factor bHLH145	Q9FGB0	DNA binding	Arabidopsis thaliana	35.2/5.1	18.3/5.1	62	28	10/63
92	DNA-directed RNA polymerase subunit beta	B1VKH5	DNA-directed RNA polymerase activity	Cryptomeria japonica	139.6/9.4	44.6/8.1	59	12	11/65
136	DNA polymerase	P10582	3'-5' exonuclease activity	Zea mays	108.2/8.6	55.3/8.5	58	15	8/23
	Protein synthesis
154	50S ribosomal protein L33	B2LML4	structural constituent of ribosome	Guizotia abyssinica	7.8/9.7	56.4/5.1	62	42	5/11
	Protein folding, degradation and assembly
45	Heat shock protein 81-3	P51818	ATP binding	Arabidopsis thaliana	80.2/5.0	26.7/8.2	59	19	9/85
73	Proteasome subunit alpha type-6	O48551	threonine-type endopeptidase activity	Glycine max	27.5/5.8	28.3/6.1	58	31	6/62
	Transport
23	NAD(P)H-quinone oxidoreductase subunit H, chloroplastic OS	A6MMH1	oxidoreductase activity, acting on NADH or NADPH	Chloranthus spicatus	45.8/5.4	18.5/7.3	75	26	12/66
52	Ras-related protein ARA-4	P28187	GTP binding	Arabidopsis thaliana	24.1/5.0	28.6/8.4	61	28	10/101
	Stress Related
62	Putative F-box/kelch-repeat protein At4g19330	O65704	N/A	Arabidopsis thaliana	62.9/7.1	27.3/5.7	59	25	10/82
151	Putative F-box/kelch-repeat protein At4g19330	O65704	N/A	Arabidopsis thaliana	62.8/7.1	44.3/4.8	65	21	8/75
	Development
53	3-ketoacyl-CoA synthase 5	Q9C6L5	fatty acid elongase activity	Arabidopsis thaliana	56.3/9.0	27.7/8.1	61	40	12/19
81	Cytoplasmic dynein 2 heavy chain 1	Q9SMH5	ATP binding	Chlamydomonas reinhardtii	483.5/6.1	40.0/8.6	58	7	22/48
	Unknown
8	Embryonic abundant protein VF30.1 OS = Vicia faba PE = 2 SV = 1	P21745	N/A	Vicia faba	30.1/6.4	18.1/4.2	67	33	7/67

^aAs indicated in Figure 2. ^bSwissprot database accession number. ^cMolecular functions were inferred from those reported in the UniProt database. ^dTheoretical isoelectric point and molecular mass. ^eExperimental isoelectric point and molecular mass. ^fMascot score. ^gSequence coverage. ^hNumber of matched and unmatched peptides.

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ISSN: 1477-5956

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