Figure 1

Representative 2-DE gel map of FFPE adenoma parathyroid tissue proteins. After deparaffinization in xylene and rehydration through a series of graded alcohols, tissue was resuspended in 20 mM TrisHCl at pH 4 (A), 6 (B) or 9 (C) with 2% SDS and 0.2 M Glycine. The homogenate was heated at 100°C for 20 min and at 60°C for 2 hours. A total of 150 μg of proteins was separated by 2-DE using 18 cm pH 3-10 NL strip and 12.5% SDS-PAGE. Proteins were detected by silver staining. The map was analyzed by Image master 2D platinum software. The gels were performed in triplicate. The reported gel images are representative of a triplicate for each pH buffer.