Figure 4

Western blot analysis. The presence of PRDX-6, BSPRY, Calmodulin, and Parvalbumin were assessed by immunoblot analysis. Aliquots of protein extracts from FFPE and fresh/frozen adenoma tissues were separated by 1-D SDS-PAGE (12% resolving gel). Proteins were transferred onto nitrocellulose membranes and incubated with specific antibodies. Immunoreactions were evidenced by ECL system.