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Table 1 Protein spots identified by LC-ESI-MS/MS from FFPE extracts separated by the SDS-out gel-free approach with probability over 95%.

From: Evaluation of formalin-fixed paraffin-embedded tissues in the proteomic analysis of parathyroid glands

Protein name

Accession No

Gene Name

Theoretical

Matched peptides

Coverage (%)

14-3-3 protein ipsilon

P62258

YWHAE

29

4.63

2

9.4

2-oxoglutarate dehydrogenase E1 component, mitochondrial

Q02218

OGDH

116

6.39

2

2.2

3-hydroxyisobutyrate dehydrogenase, mitochondrial

P31937

HIBADH

35

8.38

2

7.1

40S ribosomal protein S5

P46782

RPS5

23

9.73

2

14

40S ribosomal protein S7

P62081

RPS7

22

10.09

2

18

60S acidic ribosomal protein P2

P05387

RPLP2

12

4.42

2

24

ADP/ATP translocase 1

P12235

SLC25A4

33

9.78

2

12

Aspartate aminotransferase, cytoplasmic

P17174

GOT1

46

6.53

2

9.4

ATP synthase subunit b, mitochondrial

P24539

ATP5F1

29

9.37

2

7.8

ATP synthase subunit e, mitochondrial

P56385

ATP5

8

9.34

2

26

Calcium-binding mitochondrial carrier protein Aralar1

O75746

SLC25A12

75

8.57

3

4.9

Creatine kinase B-type

P12277

CKB

43

5.34

3

10

Cytochrome b-c1 complex subunit Rieske-like protein 1

P0C7P4

UQCRFSL1

31

9.04

3

17

Cytochrome c oxidase subunit 4 isoform 1, mitochondrial

P13073

COX4I1

20

9.52

2

16

D-3-phosphoglycerate dehydrogenase

O43175

PHGDH

57

6.29

3

6.2

Decorin

P07585

PE

40

8.75

2

11

Elongation factor 1-alpha 1

P68104

EEF1A1

50

9.10

2

5.2

Elongation factor 2

P13639

EEF2

95

6.41

2

2.9

Enoyl-CoA hydratase, mitochondrial

P30084

ECHS1

31

8.34

2

13

Heterogeneous nuclear ribonucleoprotein H

P31943

HNRNPH1

49

6.74

2

7.6

Isochorismatase domain-containing protein 2, mitochondrial

Q96AB3

ISOC2

22

7.67

4

46

Keratin, type I cytoskeletal 16

P08779

KRT16

51

4.98

5

18

Keratin, type II cytoskeletal 2 epidermal

P35908

KRT2

66

8.07

5

13

Keratin, type II cytoskeletal 6B

P04259

KRT6B

60

8.09

3

11

L-lactate dehydrogenase B chain

P07195

LDHB

37

5.71

3

10

Lumican

P51884

LUM

38

6.16

4

12

Lysosome-associated membrane glycoprotein 1

P11279

LAMP1

45

9.00

2

5.0

Malate dehydrogenase, cytoplasmic

P40925

MDH1

36

6.91

2

8.7

Medium-chain specific acyl-CoA dehydrogenase, mitochondrial

P11310

ACADM

47

8.61

2

8.3

Mitochondrial 2-oxoglutarate/malate carrier protein

Q02978

SLC25A11

34

9.92

5

18

NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 4

O00483

NDUFA4

9

9.42

2

25

Nucleoside diphosphate kinase

Q32Q12

NME1-NME2

33

8.70

2

6.80

Peroxiredoxin-6

P30041

PRDX6

25

6.00

3

17

Proteasome activator complex subunit 1

Q06323

PSME1

29

5.78

2

9.2

Protein kinase C inhibitor-2

Q8WYJ5

 

14

6.50

3

41

Pyruvate dehydrogenase E1 subunit alpha somatic form mitochondrial

P08559

PDHA1

43

8.35

3

7.9

Ras-related protein Rab-1A

P62820

RAB1A

23

5.93

2

13

Serotransferrin

P02787

TF

77

6.81

2

4.7

Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial

P31040

SDHA

73

7.06

2

6.6

Transitional endoplasmic reticulum ATPase

P55072

VCP

89

5.14

3

5.7