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Table 2 Protein spots identified by LC-ESI-MS/MS from FFPE extracts separated by the SDS-out + TCA/Acetone gel-free approach with probability over 95%.

From: Evaluation of formalin-fixed paraffin-embedded tissues in the proteomic analysis of parathyroid glands

Protein name

Accession No

Gene Name

Theoretical

Matched peptides

Coverage

(%)

   

MW

pI

SDS-Out +TCA

SDS-Out +TCA

14-3-3 protein theta

P27348

YWHAQ

28

4.68

2

9.0

14-3-3 protein zeta/delta

P63104

YWHAZ

28

4.73

2

11

39S ribosomal protein L39, mitochondrial

Q9NYK5

MRPL39

39

7.56

2

7.7

60S ribosomal protein L7

P18124

RPL7

29

1066

2

8.5

Adenine phosphoribosyltransferase

P07741

APRT

20

5.78

3

20

Adenylate kinase 2, mitochondrial

P54819

AK2

26

7.67

2

11

Aflatoxin B1 aldehyde reductase member 2

O43488

AKR7A2

40

6.70

2

9.5

Cathepsin G

P08311

CTSG

29

11.19

2

11

cDNA FLJ61525, highly similar to Thyroglobulin

B4E153

 

98

5.50

2

17

Fructose-bisphosphate aldolase A

P04075

ALDOA

39

8.30

4

9.9

Fumarate hydratase, mitochondrial

P07954

FH

55

8.85

2

6.3

Galectin-3-binding protein

Q08380

LGALS3BP

65

5.13

2

4.4

G-protein gamma-12 subunit

Q69YP5

DKFZp762E193

8

9.14

2

38

Guanine nucleotide-binding protein subunit beta-2-like 1

P63244

GNB2L1

35

7.60

2

8.8

Heat shock protein HSP 90-alpha

P07900

HSP90AA1

85

4.94

2

5.1

Ig kappa chain C region

P01834

IGKC

12

5.58

3

48

L-xylulose reductase

Q7Z4W1

DCXR

26

8.33

2

9.4

Macrophage-capping protein

P40121

CAPG

39

5.82

2

10

Mitochondrial import inner membrane translocase subunit Tim8 A

O60220

TIMM8A

11

5.08

2

23

NAD(P) transhydrogenase, mitochondrial

Q13423

NNT

114

8.31

2

2.5

NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial

O75489

NDUFS3

30

6.98

2

9.8

Poly(rC)-binding protein 1

Q15365

PCBP1

37

6.66

2

6.7

Profilin-1

P07737

PFN1

15

8.44

3

23

Puromycin-sensitive aminopeptidase

P55786

NPEPPS

103

5.49

2

2.7

Sideroflexin-1

Q9H9B4

SFXN1

36

9.22

2

17

Transaldolase

P37837

TALDO1

38

6.37

2

7.1

Trifunctional enzyme subunit beta, mitochondrial

P55084

HADHB

51

9.45

2

4.6

Ubiquitin

P62988

RPS27A

9

6.56

2

33

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