Figure 13

Cardiogenol C inhibits chromatin remodeling gene expression in HBPCs. (A) Semi-quantitative RT-PCR analysis of three components of the polycomb gene complex (Phc1, Ezh2 and YY1). Expressions of all three genes were down-regulated in HBPCs following Cardiogenol C-treatment. The bar chart showed the normalized density measurements of the PCR bands. (B and C) Western blot analyses confirmed that Cardiogenol C inhibited Phc1and Ezh2 expressions in HBPCs. α-tubulin served as an internal control. The bar charts showed the normalized density measurements of the protein bands. The experiments were performed in triplicates, and each data point was expressed as mean value ± SEM. *Statistically significant difference (p < 0.05).