Table 1 Primers for semi-quantitative RT-PCR analysis
Gene | Â | Primer sequences (5' to 3') | Product size (bp) | Â |
|---|---|---|---|---|
β-actin | Sense | 5'-TGT TAC CAA CTG GGA CGA C-3' | 573 |  |
| Â | Anti-sense | 5'-AAG GAA GGC TGG AAA AGA G-3' | Â | Â |
CD34 | Sense | 5'-TAG CCC AGT CTG AGG TTA GG-3' | 205 | Â |
| Â | Anti-sense | 5'-AGC AGA ACT CCA GAG GTG AC-3' | Â | Â |
Dkk1 | Sense | 5'-TGT CTA GGG GGT CGA ATG TA-3' | 300 | Â |
| Â | Anti-sense | 5'-CAC AGA GCC CCA AAT GTA TC-3' | Â | Â |
Etv6 | Sense | 5'-GTC TCC TTC AGC AGA AAC CA-3' | 424 | Â |
| Â | Anti-sense | 5'-CTG ATC CCA ACT GTG TGT CA-3' | Â | Â |
GATA4 | Sense | 5'-GTG GTG GGT TTT TCT TTG TG-3' | 443 | Â |
| Â | Anti-sense | 5'-CAT AGT CAC CAA GGC TGC TT-3' | Â | Â |
Hes6 | Sense | 5'-AGG GTA GCA GCT TTC AGG AT-3' | 354 | Â |
| Â | Anti-sense | 5'-CCT GAG CTG TCT CCA CCT TA-3' | Â | Â |
Keratin 5 | Sense | 5'-GGA CCA GTC AAC ATC TCT GT-3' | 378 | Â |
| Â | Anti-sense | 5'-TGC CTA AAA GAA GCA GTC TC-3' | Â | Â |
Keratin 14 | Sense | 5'-GAA TGG TTC TTC AGC AAG AC-3' | 386 | Â |
| Â | Anti-sense | 5'-ATG AGG AGA ATT GGG AAG AT-3' | Â | Â |
Kremen1 | Sense | 5'-GCA TCC ATT TCA ACT TCA CC-3' | 431 | Â |
| Â | Anti-sense | 5'-CGT ACA CAG TCC ATC CTT CC-3' | Â | Â |
Mthfr | Sense | 5'-CGT AGA GCA ACT TAG GGA GA-3' | 314 | Â |
| Â | Anti-sense | 5'-CCC ATG AGA AGA ACT AGC AG-3' | Â | Â |
Nestin | Sense | 5'-GAA TCA GAT CGC TCA GAT CC-3' | 487 | Â |
| Â | Anti-sense | 5'-GCA CGA CAC CAG TAG AAC TGG-3' | Â | Â |
Nkx-2.5 | Sense | 5'-ATC CTA AAC CTG GAG CAG CA-3' | 501 | Â |
| Â | Anti-sense | 5'-AGA TCT TGA CCT GCG TGG AC-3' | Â | Â |
Osteocalcin | Sense | 5'-GCA GCT TGG TGC ACA CCT AG-3' | 405 | Â |
| Â | Anti-sense | 5'-ACC TTA TTG CCC TCC TGC TT-3' | Â | Â |
Phc1 | Sense | 5'-AGC AGA CTT TGC AGA GAA GA-3' | 212 | Â |
| Â | Anti-sense | 5'-TAT ACT GGC TGG GGA TCC TG-3' | Â | Â |
Plod2 | Sense | 5'-GCC AGA AGG TGA GAT TAC TG-3' | 397 | Â |
| Â | Anti-sense | 5'GCA CAA CAG GGT ACT TGT CT-3' | Â | Â |
PPAR-γ | Sense | 5'-ACT GTC GGT TTC AGA AGT GC-3' | 258 |  |
| Â | Anti-sense | 5'-GAT CTC TTG CAC GGC TTC TA-3' | Â | Â |
Sik1 | Sense | 5'-GGA GAG GAA AGG AGA CAC TT-3' | 296 | Â |
| Â | Anti-sense | 5'-GGA TAC TGA GTG CTT GCT TC-3' | Â | Â |
Sox2 | Sense | 5'-GCC CTG CAG TAC AAC TCC AT-3' | 363 | Â |
| Â | Anti-sense | 5'-GTC ATT TGC TGT GGG TGA TG-3' | Â | Â |
Srms | Sense | 5'-TCC CTA TGA AGG AAT GAC CA-3' | 354 | Â |
| Â | Anti-sense | 5'-AGC CTG TGT CAG TGA AGG AG-3' | Â | Â |
Tbx5 | Sense | 5'-CAA AGA CAG GTC TTG CGA TT-3' | 426 | Â |
| Â | Anti-sense | 5'-GGT GAG TTT GAG CTT CTG GA-3' | Â | Â |
Tcf3 | Sense | 5'-CTT ATC CCC CAA CTA CGA TG-3' | 315 | Â |
| Â | Anti-sense | 5'-ATA GGA GTC GGG AGG TCT CT-3' | Â | Â |
Wnt11 | Sense | 5'-AGG ATC CCA AGC CAA TAA AC-3' | 330 | Â |
| Â | Anti-sense | 5'-GGA TCC CAC CTT CTC ATT CT-3' | Â | Â |
YY1 | Sense | 5'-GCG GCA AGA AGA GTT ACC TG-3' | 418 | Â |
| Â | Anti-sense | 5'-GGT GTG CAG ATG CTT TCT CA-3' | Â | Â |
Ezh2 | Sense | 5'-AGG CTA ATT GGG ACC AAA AC-3' | 306 | Â |
| Â | Anti-sense | 5'-AAA GCG GTT TTG ACA CTC TG-3' | Â | Â |