Figure 4

The first-dimensional and second-dimensional immunoblotting analysis of ATP-β in leaves of rice under heat stress. The proteins extracted from leaves of rice under heat stress were separated in 1D and 2-DE gels, transferred to an NC membrane, and then incubated with anti-ATP-β antibody. A: Proteins on the 1D gel were probed with an anti-ATP-β antibody. B: Proteins on the 1D gel were visualized by Coomassie blue staining. C: Proteins on the 2-DE gel were visualized by Coomassie blue staining. D: Proteins on the 2-DE gel were visualized by Pro-Q Diamond staining. E: Proteins on the 2-DE gel were probed by anti-ATP-β antibody. 1: Proteins extracted from the leaves of rice under 24 h heat stress. 2: Proteins extracted from the leaves of rice under 12 h heat stress. 3: Proteins extracted from the leaves of rice under normal conditions (Control). M: Marker.