Figure 2

Silver stained 2D-PAGE of crude mare follicular fluid. 100 μg of proteins were applied to a non-linear IPG strip (pI 3-10) in the first dimension and separated on a SDS-PAGE (10%) gel in the second dimension (Molecular Weight: 10-200 kDa range). The positions of the high-abundance proteins albumin (ALB) and immunoglobulin heavy chain (IGHC1) are indicated by dark dotted rectangles or dark circles. All other identified proteins are named and their characteristics are shown in Table 1.