Proteomic analysis of mare follicular fluid during late follicle development

Table 1 Identified proteins of crude mare follicular fluid separated by 2D-PAGE (pI: 3-10; MW: 10-200; silver staining)

Gene	Accession N° ¹	Protein name	Score ²	Theoretical		Peptide N° ³	Sequence coverage (%) ⁴
				pI ³	Mr (Da) ³
A1BG	gi\|194216172	Alpha-1-B glycoprotein	374	8.74	68222	10	21
A2M	gi\|194211675	Alpha-2-macroglobulin	1389	6.24	163911	21	25
AGT	gi\|194206059	Angiotensinogen	50	7.06	70056	1	3
ALB	gi\|76363596	Albumin	294	5.89	68494	31	43
ALB	gi\|76363596	Albumin	112	5.89	68494	2	4
ALB	gi\|399672	Preproalbumine	91	5.95	68554	2	4
APOA1	gi\|3915607	Apolipoprotein A-I	105	5.2	30178	2	7
APOH	gi\|149723623	Apolipoprotein H	33	8.43	38511	1	2
AT3	gi\|179161	Antithrombin III	92	6.32	52585	2	5
DNAH7	gi\|149699076	Similar to dynein, axonemal, heavy polypeptide 7 isoform 1	232	5.5	61446	5	11
CFB	gi\|149732066	Complement factor B	141	6.75	85930	3	4
CP	gi\|180249	Ceruloplasmin	48	5.29	97637	1	1
DBP	gi\|73975213	Vitamin D-binding protein	39	5.2	52940	1	1
FGA	gi\|3789960	Fibrinogen alpha	57	6.45	49477	2	4
HP	gi\|149699777	Haptoglobin precursor	103	6.24	45176	5	9
HP	gi\|149699777	Haptoglobin precursor	112	5.59	38441	3	9
HPX	gi\|386789	Hemopexin	32	6.57	51512	1	1
IgG	gi\|9858135	Immunoglobulin G1b	70	5.7	46904	2	10
IGHC1	gi\|15020816	Immunogobulin gamma 1	30	7.68	37415	1	2
IGHC1	gi\|15020816	Immunogobulin gamma 1	30	7.68	37415	1	2
IGHC1	gi\|15020816	Immunogobulin gamma 1	30	7.68	37415	1	2
IGHC1	gi\|15020816	Immunogobulin gamma 1	30	7.68	37415	1	2
LF	gi\|13431954	Lactoferrine	54	8.66	77162	1	3
A1AT	gi\|194225326	Alpha-1-antitrypsin	45	5.23	46913	2	3
A1AT	gi\|194225326	Alpha-1-antitrypsin	398	5.31	46896	5	13
A1AT	gi\|194225326	Alpha-1-antitrypsin	83	5.23	46913	3	5
TF	gi\|3892525	Transferrin	219	8.6	6880	4	42

¹ Accession N° is an accession number from the NCBInr databases. ² Score is -10*Log (P), where P is the probability that the observed match is a random event, it is based on NCBInr database using the MASCOT searching program. ³ PI: isoelectric point; Mr: Molecular weight; Peptid N°: Peptid number. ⁴ Sequence coverage (%) is based on number of peptide masses matched in NCBInr databases.

ISSN: 1477-5956