Proteomic analysis of mare follicular fluid during late follicle development

Table 2 Identified proteins of mare follicular fluid after depletion with MARS-6 and separation by 2D-PAGE (pI: 3-10; MW: 10-200; silver staining)

Gene	Accession N° ¹	Protein name	Score ²	Theoretical		Peptide N° ³	Sequence coverage (%) ⁴
				pI ³	Mr (Da) ³
A1AT	gi\|194225326	Alpha-1-antitrypsin	398	5.31	46896	6	13
A1BG	gi\|194216172	Alpha-1B-glycoprotein	374	8.74	68222	8	21
A2M	gi\|194211675	Alpha-2-macroglobulin	1406	6.24	163911	25	26
AGT	gi\|194206059	Angiotensinogen	50	7.06	70056	1	3
ALB	gi\|76363596	Serum albumin precursor	112	5.89	68494	2	4
APOH	gi\|149723623	Apolipoprotein H	33	8.43	38511	1	2
CFB	gi\|149732066	B-factor, properdin	139	6.75	85930	2	4
DNAH7	gi\|149699076	Dynein, axonemal, heavy polypeptide 7 isoform 1	232	5.5	61446	5	11
HP	gi\|149699777	Haptoglobin precursor	112	5.59	38441	3	9
HPX	gi\|149719403	Hemopexin	122	7.58	51324	3	6
HRG	gi\|194222677	Histidine-rich glycoprotein	245	7.64	42955	5	16
IGHC1	gi\|15020816	Immunogobulin gamma 1 heavy chain constant region	31	7.68	37415	1	2

¹ Accession N° is an accession number from the NCBInr databases.² Score is -10*Log (P), where P is the probability that the observed match is a random event, it is based on NCBInr database using the MASCOT searching program. ³ PI: isoelectric point; Mr: Molecular weight; Peptid N°: Peptid number. ⁴ Sequence coverage (%) is based on number of peptide masses matched in NCBInr databases.

ISSN: 1477-5956