Proteomic analysis of mare follicular fluid during late follicle development

Fahiminiya, Somayyeh; Labas, Valérie; Roche, Stéphane; Dacheux, Jean-Louis; Gérard, Nadine

doi:10.1186/1477-5956-9-54

Proteome Science

Table 3 Identified proteins of mare follicular fluid after enrichment by hexapeptide ligand library and separation by 2D-PAGE (pI: 3-10; MW: 10-200; silver staining)

From: Proteomic analysis of mare follicular fluid during late follicle development

Gene	Accession N° ¹	Protein name	Score ²	Theoretical		Peptide N° ³	Sequence coverage (%) ⁴
				pI ³	Mr (Da) ³
ACT	gi\|6626	Actin	55	5.29	41779	1	2
ALB	gi\|126723507	Preproalbumin	188	5.95	68554	4	7
ALB	gi\|2492797	Albumin precursor	28	5.85	67837	1	1
AMBP	gi\|149738520	AMBP protein precursor	101	6.82	38985	2	5
AMY2A	gi\|178585	Alpha-amylase	78	6.32	57714	1	3
AMY2A	gi\|178585	Alpha-amylase	37	6.32	57714	1	3
AMY2A	gi\|178585	Alpha-amylase	106	6.32	57714	1	3
APOA1	gi\|149716548	Apolipoprotein A-I precursor	883	5.66	30312	13	50
APOA1	gi\|149716548	Apolipoprotein A-I precursor	675	5.66	30312	13	44
APOA1	gi\|149716548	Apolipoprotein A-I precursor	900	5.66	30312	17	62
APOA1	gi\|149716548	Apolipoprotein A-I precursor	281	5.66	30312	6	25
C3	gi\|73987236	Complement C3 precursor	85	6.95	173988	1	1
C3	gi\|116597	Complement C3 precursor	53	6.12	186342	1	0
C3	gi\|554423	Complement component C3	179	5.73	31844	3	17
C3	gi\|554423	Complement component C3	86	5.73	31844	2	8
CLU	gi\|126352584	Clusterin	43	5.77	52121	1	2
CLU	gi\|126352584	Clusterin	248	5.77	52121	3	12
CLU	gi\|126352584	Clusterin	240	5.77	52121	4	12
FCN1	gi\|194226003	Ficolin 1	156	5.37	34486	4	14
FGA	gi\|3789960	Fibrinogen A-alpha chain	65	6.45	49477	1	2
FGA	gi\|3789960	Fibrinogen A-alpha chain	65	6.45	49477	2	4
FGA	gi\|3789960	Fibrinogen A-alpha chain	695	6.45	49477	10	37
FGB	gi\|149698358	Fibrinogen beta chain precursor	502	8.53	55398	6	26
FGB	gi\|149698358	Fibrinogen beta chain precursor	409	8.53	55398	8	28
FGB	gi\|149698358	Fibrinogen beta chain precursor	137	8.53	55398	2	6
FGB	gi\|149698358	Fibrinogen beta chain precursor	125	8.53	55398	2	6
FGG	gi\|1916266	Fibrinogen-gamma	69	4.78	8740	2	62
FH	gi\|194227377	Complement regulator factor H	95	7.52	140649	2	1
GAPDH	gi\|31645	Glyceraldehyde-3-phosphate dehydrogenase	38	8.26	36031	1	4
GPX	gi\|7262515	glutathione peroxidase	144	5.78	8893	2	37
HRG	gi\|194222677	Histidine-rich glycoprotein	227	7.64	43003	4	12
PSP	gi\|126352355	Parotid secretory protein	79	4.75	26882	2	9
SNX1	gi\|149711176	Nexin-1 isoform 1	75	9.53	44065	1	3
UN	gi\|28317	Unnamed protein product	84	5.17	59492	1	2

¹ Accession N° is an accession number from the NCBInr databases.² Score is -10*Log (P), where P is the probability that the observed match is a random event, it is based on NCBInr database using the MASCOT searching program. ³ PI: isoelectric point; Mr: Molecular weight; Peptid N°: Peptid number⁴ Sequence coverage (%) is based on number of peptide masses matched in NCBInr databases.

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ISSN: 1477-5956

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