Proteomic analysis of mare follicular fluid during late follicle development

Table 4 Overview of studies performed on follicular fluid of four different species

Study	Follicular fluid samples	Part of sample analyzed	Separation method	MS method
This study	Mare (MFF)	crude, depleted and enriched mare follicular fluid from normal follicles	2D-PAGE 1D-PAGE	nano-LC-MS/MS
Fahiminiya et al. (2010)	Canine (CFF)	crude canine follicular fluid from normal follicles	2D-PAGE	nano-LC-MS/MS
Angelucci et al. (2006)	Human (HFF)	crude follicular fluid from normo-ovulatory women undergoing assisted reproduction techniques due to a male infertility factor	2D-PAGE	MALDI-TOF-MS
Jarkovska et al. (2010)	Human (HFF)	depleted follicular fluid of women undergoing successful IVF	2D-PAGE ProteomeLab PF 2D	MALDI-TOF-MS
Bijttebier et al. (2009)	Porcine (PFF)	crude porcine follicular fluid of normal follicles	iTRAQ labeling	LC ESI-Q-TOF MS/MS

For each study the following information is presented: 1) follicular fluid derived from specific species 2) the nature of the samples, used in the study (physiological status and whether the study focused on depleted, enriched or crude follicular fluid) 3) the methods used to separate proteins and peptides and 4) MS method used for analysis.

ISSN: 1477-5956